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IL-22 (pig) ELISA kit

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    IL-22 (pig) ELISA kit
  • IL-22 (pig) ELISA kit
Cat No: A05424
Assays - Hrp Kits
Price is excluding VAT and does not include packaging neither shipping

Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...

: 96 wells
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Territorial Availability: Available worldwide directly through Bertin Technologies or your local distributor
Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Interleukin 22 (IL-22) is one of the 9 members of the IL-10 family(1) (IL-10/IL-19/IL-20/IL-22/IL-24/IL-26/IL-28A/IL-28B and IL-29).
IL-22 is a polypeptide of 146 amino acids with a molecular weight of 16.7 kDa(2). IL-22 structure contains six antiparallel helices(3). It is produced at a monomeric state(4) by cells from the lymphoid lineage(5) (CD4+ T cells, Th22 subpopulation, NK cells) . This cytokine acts only on non-hematopoietic stromal cells, in particular epithelial cells, keratinocytes, and hepatocytes(6).
IL-22 increases the innate immunity of tissue cells (induction of antimicrobial molecules), protects tissues from damage, and enhances their regenerations(3).
Upregulation is find in many diseases like psoriasis and Crohn's disease.
Size: 96 wells
Shipping: wet ice
Stability: Store at +4degrees; shelf life 6months maximum after production
Application Media: It is the responsibility of the user to check the compatibility of the assay with the study matrix
Sample volume: 100 µL
Tracer: horseradish peroxidase HRP
Detection Limit: ≤0,02 ng/mL
Standard Curve Range: 0,02-2,00 ng/mL
Custom Code: 3822000000
UNSPSC code: 41116104

1. Sabat R IL-10 family of cytokines. 2010, Cytokine Growth Factor Rev 21:315–324

2. Xie MH, Aggarwal S, Ho WH et al. Interleukin (IL)-22, a novel human cytokine that signals through the interferon receptor-related proteins CRF2–4 and IL-22R. 2000, J Biol Chem 275:31335–31339

3. Robert Sabat, Ellen Witte, Katrin Witte, and Kerstin Wolk IL-22 and IL-17: An Overview. 2013, IL-17, IL-22 and Their Producing Cells: Role in Inflammation and Autoimmunity, 2nd ed., pp.11-35

4. Zdanov A Structural analysis of cytokines comprising the IL-10 family. 2010, Cytokine Growth Factor Rev 21:325–330

5. Trifari S, Kaplan CD, Tran EH et al. Identification of a human helper T cell population that has abundant production of interleukin 22 and is distinct from T(H)-17, T(H)1 and T(H)2 cells. 2009, Nat Immunol 10:864–871

6. Rutz S, Eidenschenk C, Ouyang W IL-22, not simply a Th17 cytokine. 2013, Immunol Rev. Mar;252(1):116-32

SPI-Bio is a brand name of Bertin Bioreagent.
Bertin Bioreagent includes over 100 employees, chemists, biochemists, immunoanalysts, pharmacists and technicians.
Always at the forefront of innovation and after launching on the market products incorporating in their time innovative technologies (Acetylcholinesterase tracer for EIA), this brand offers a wide and unique range of assays for biomarker Immunoassays (SPI-Bio TM) thanks to a solid knowledge acquired during the last 25 years in developping over 100 biomarkers assays.

To better serve you, we aim to design our products and reagents to address your needs on the expected performance in terms of a possible range of use, but also on operational conditions of use & regulations.
Thanks to our production processes and quality controls, we provide you with products with compliance to specifications.
Our technical support & science teams are available to help you assess their relevance depending on your needs, to specify the conditions of application and their fields of use. Finally, our researchers are ready to provide you with the necessary support by going, if needed, in your laboratories to validate your operating conditions and to solve possible problems.

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