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CXCL10 (pig) ELISA kit

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    CXCL10 (pig) ELISA kit
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  • CXCL10 (pig) ELISA kit
Cat No: A05419
Assays - Hrp Kits
SPI-Bio
€390.00
Price is excluding VAT and does not include packaging neither shipping

Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...

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: 96 wells
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Territorial Availability: Available worldwide directly through Bertin Technologies or your local distributor
Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
CXCL10 is also known as Interferon gamma-induced protein 10 (IP-10) or small-inducible cytokine B10 with a molecular weight close to 10 kDa. CXCL10 is expressed by several cellular types like monocytes, endothelial cells and fibroblasts stimulated by the interferon-gamma (INF-γ). The receptor of this chimiokine is CXCR3 that is shared with CXCL9 and CXCL11. CXCL-10 plays an important role in the recruitment of the immune cells to sites of inflammation. It is included in physiological process like bone marrow colony formation and angiogenesis but also in some disorders like Th1-type human inflammatory diseases, or cancer.
Size: 96 wells
Shipping: wet ice
Stability: Store at +4degrees; shelf life 6months maximum after production
Application Media: It is the responsibility of the user to check the compatibility of the assay with the study matrix
Sample volume: 100 µL
Tracer: horseradish peroxidase HRP
Detection Limit: ≤0,08 ng/mL
Standard Curve Range: 0,08-10,00 ng/mL
Custom Code: 3822000000
UNSPSC code: 41116104

Luster A. D., Unkeless J. C., Ravetch J. V. Gamma-interferon transcriptionally regulates an early-response gene containing homology to platelet proteins. 1985 Nature 315:672.

Booth V,. Keizer D.W., Kamphuis M.B., et al. The CXCR3 binding chemokine IP-10/CXCL10: structure and receptor interactions. 2002 Biochemistry. 41 (33): 10418–25.

Dufour J. H., Dziejman M., Liu M. T., et al. IFN-γ-Inducible Protein 10 (IP-10; CXCL10)-Deficient Mice Reveal a Role for IP-10 in Effector T Cell Generation and Trafficking. 2002 J Immunol 168:3195-3204

Gottlieb, A. B., Luster A. D., Posnett D. N., et al. Detection of a γ-interferon-induced protein IP-10 in psoriatic plaques. 1988 J. Exp. Med. 168:941-948.

Angiolillo A.L., Sgadari C., Taub D.D., et al. Human interferon-inducible protein 10 is a potent inhibitor of angiogenesis in vivo. 1995 The Journal of Experimental Medicine. 182 (1): 155–162.

SPI-Bio is a brand name of Bertin Bioreagent.
Bertin Bioreagent includes over 100 employees, chemists, biochemists, immunoanalysts, pharmacists and technicians.
Always at the forefront of innovation and after launching on the market products incorporating in their time innovative technologies (Acetylcholinesterase tracer for EIA), this brand offers a wide and unique range of assays for biomarker Immunoassays (SPI-Bio TM) thanks to a solid knowledge acquired during the last 25 years in developping over 100 biomarkers assays.

To better serve you, we aim to design our products and reagents to address your needs on the expected performance in terms of a possible range of use, but also on operational conditions of use & regulations.
Thanks to our production processes and quality controls, we provide you with products with compliance to specifications.
Our technical support & science teams are available to help you assess their relevance depending on your needs, to specify the conditions of application and their fields of use. Finally, our researchers are ready to provide you with the necessary support by going, if needed, in your laboratories to validate your operating conditions and to solve possible problems.

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