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IL-4 (pig) ELISA kit

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    IL-4 (pig) ELISA kit
  • IL-4 (pig) ELISA kit
Cat No: A05420
Assays - Hrp Kits
Price is excluding VAT and does not include packaging neither shipping

Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...

: 96 wells
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Territorial Availability: Available worldwide directly through Bertin Technologies or your local distributor
Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Discovered in 1992, Interleukin-4 (IL-4) is a monomeric glycosylated polypeptide with a molecular weight of 18-20 kDa. IL-4 structure contains four alpha-helices which are anti-parallel and two anti-parallel beta-sheets.
IL-4 is also known as B-cell stimulatory factor 1 or B-cell growth factor 1 and as Lymphocyte stimulatory factor. IL-4 is a key regulator in humoral and adaptive immunity. IL-4 plays an essential role by promoting Th2 cell differentiation while inhibiting Th1 cell differentiation. IL-4 is an anti-apoptosis factor for the immune and non-immune cells. Due to its main function, IL-4 is an anti-inflammatory cytokine in autoimmune diseases. IL-4 deregulation could be a factor in the development of some diseases like allergic diseases or in the treatment resistance of tumors
Size: 96 wells
Shipping: wet ice
Stability: Store at +4degrees; shelf life 6months maximum after production
Application Media: It is the responsibility of the user to check the compatibility of the assay with the study matrix
Sample volume: 100 µL
Tracer: horseradish peroxidase HRP
Detection Limit: ≤0,02 ng/mL
Standard Curve Range: 0,02-2,00 ng/mL
Custom Code: 3822000000
UNSPSC code: 41116104

1. Stephen T. Smiley, Michael J. Grusby, Interleukin 4, 1998, Encyclopedia of Immunology 1451–1453

2. Carr C, Aykent S, Kimack NM, Levine AD, Disulfide assignments in recombinant mouse and human interleukin 4, 1991, Biochemistry. 30 (6): 1515–23-3. Walter MR, Cook WJ, Zhao BG et al, Crystal structure of recombinant human interleukin-4, 1992, J. Biol. Chem. 267 (28): 20371–6

4. J. Zamorano, M.D. Rivas, M. Pérez-G, Interleukin-4: A multifunctional cytokine, 2003, Inmunología Vol. 22/Num 2/215-224

5. Feldmann M, Brennan FM, Maini RN, Role of cytokines in rheumatoid arthritis, 1996, Annu.Rev Immunol; 14: 397-440

6. Zamorano J, Kelly AE, Austrian J et al, Costimulation of resting B lymphocytes alters the IL-4-activated IRS2 signaling pathway in a STAT6 independent manner: implications for cell survival and proliferation, 2011 Cell Res. 11: 44-54

SPI-Bio is a brand name of Bertin Bioreagent.
Bertin Bioreagent includes over 100 employees, chemists, biochemists, immunoanalysts, pharmacists and technicians.
Always at the forefront of innovation and after launching on the market products incorporating in their time innovative technologies (Acetylcholinesterase tracer for EIA), this brand offers a wide and unique range of assays for biomarker Immunoassays (SPI-Bio TM) thanks to a solid knowledge acquired during the last 25 years in developping over 100 biomarkers assays.

To better serve you, we aim to design our products and reagents to address your needs on the expected performance in terms of a possible range of use, but also on operational conditions of use & regulations.
Thanks to our production processes and quality controls, we provide you with products with compliance to specifications.
Our technical support & science teams are available to help you assess their relevance depending on your needs, to specify the conditions of application and their fields of use. Finally, our researchers are ready to provide you with the necessary support by going, if needed, in your laboratories to validate your operating conditions and to solve possible problems.

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