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IL-17A (pig) ELISA kit

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    IL-17A (pig) ELISA kit
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  • IL-17A (pig) ELISA kit
Cat No: A05423
€395.00
Price is excluding VAT and does not include packaging neither shipping

Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...

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: 96 wells
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Territorial Availability: Available worldwide directly through Bertin or your local distributor
Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
Synonyms:
Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Interleukin-17A (IL-17A) is one of the six members of the IL-17 family(1) (from IL-17A to IL-17F).
IL-17A is a polypeptide of 132 amino acids with a molecular weight of 14.9 kDa. IL-17A structure contains 4 antiparallel strands connected by two disulfides bridges(2). It can form either a homodimer or a heterodimer with IL-17F.
Cells from the lymphoid lineage (CD4+ T cells, Th17 subpopulation, NK cells)(3-4) and Paneth cells(5) have been identified as producers of IL-17A.
IL-17A is a proinflammatory mediator, its upregulation is find in many diseases like skin disorders (psoriasis, acne inversa) or Crohn's disease.
Size: 96 wells
Shipping: wet ice
Stability: Store at +4degrees; shelf life 6months maximum after production
Application Media: It is the responsibility of the user to check the compatibility of the assay with the study matrix
Sample volume: 100 µL
Tracer: horseradish peroxidase HRP
Detection Limit: ≤0,02 ng/mL
Standard Curve Range: 0,02-2,00 ng/mL
Custom Code: 3822000000
UNSPSC code: 41116104

1. Iwakura Y, Ishigame H, Saijo S, Nakae S Functional specialization of interleukin-17 family members. 2011, Immunity 34:149–162

2. Robert Sabat, Ellen Witte, Katrin Witte, and Kerstin Wolk IL-22 and IL-17: An Overview 2013, IL-17, IL-22 and Their Produci