IL-22 (pig) ELISA kit

Assay Kits - ELISA

Zoom

Cat No: A05424

Assay Kits - Elisa

Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...

This product can not be purchased online. Please contact us

Product Overview
Data
Scientific Literature
Downloads

                        Territorial Availability: Available worldwide directly through Bertin or your local distributor
                    

                                            Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
                    

Synonyms:

Interleukin-22

Correlated keywords:

ELISA

Chemokin

Th22

epithelial cells

keratinocytes

hepatocytes

cells

Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Interleukin 22 (IL-22) is one of the 9 members of the IL-10 family(1) (IL-10/IL-19/IL-20/IL-22/IL-24/IL-26/IL-28A/IL-28B and IL-29).
IL-22 is a polypeptide of 146 amino acids with a molecular weight of 16.7 kDa(2). IL-22 structure contains six antiparallel helices(3). It is produced at a monomeric state(4) by cells from the lymphoid lineage(5) (CD4+ T cells, Th22 subpopulation, NK cells) . This cytokine acts only on non-hematopoietic stromal cells, in particular epithelial cells, keratinocytes, and hepatocytes(6).
IL-22 increases the innate immunity of tissue cells (induction of antimicrobial molecules), protects tissues from damage, and enhances their regenerations(3).
Upregulation is find in many diseases like psoriasis and Crohn's disease.

Size	96 wells
Shipping	wet ice
Specificity	refer to technical booklet
Application Media	It is the responsibility of the user to check the compatibility of the assay with the study matrix
Sample volume	100 µL
Tracer	horseradish peroxidase HRP
Detection Limit	≤0,02 ng/mL
Standard Curve Range	0,02-2,00 ng/mL
Custom Code	3822000000
UNSPSC code	41116104

1. Sabat R IL-10 family of cytokines. 2010, Cytokine Growth Factor Rev 21:315–324

2. Xie MH, Aggarwal S, Ho WH et al. Interleukin (IL)-22, a novel human cytokine that signals through the interferon receptor-related proteins CRF2–4 and IL-22R. 2000, J Biol Chem 275:31335–31339

3. Robert Sabat, Ellen Witte, Katrin Witte, and Kerstin Wolk IL-22 and IL-17: An Overview. 2013, IL-17, IL-22 and Their Producing Cells: Role in Inflammation and Autoimmunity, 2nd ed., pp.11-35

4. Zdanov A Structural analysis of cytokines comprising the IL-10 family. 2010, Cytokine Growth Factor Rev 21:325–330

5. Trifari S, Kaplan CD, Tran EH et al. Identification of a human helper T cell population that has abundant production of interleukin 22 and is distinct from T(H)-17, T(H)1 and T(H)2 cells. 2009, Nat Immunol 10:864–871

6. Rutz S, Eidenschenk C, Ouyang W IL-22, not simply a Th17 cytokine. 2013, Immunol Rev. Mar;252(1):116-32

Technical File

MSDS

/ We also advise you

Cross selling for this product : IL-22 (pig) ELISA kit There are 10 products.