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IL-22 (pig) ELISA kit

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    IL-22 (pig) ELISA kit
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  • IL-22 (pig) ELISA kit
Cat No: A05424
Assay Kits - Elisa
Bertin Bioreagent
€401.00
Price is excluding VAT and does not include packaging neither shipping

Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...

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: 96 wells

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Territorial Availability: Available worldwide directly through Bertin or your local distributor
Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
Synonyms:
  • Interleukin-22
Correlated keywords:
  • ELISA
  • EIA
  • Chemokin
  • Th22
  • epithelial cells
  • keratinocytes
  • hepatocytes
  • cells
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Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Interleukin 22 (IL-22) is one of the 9 members of the IL-10 family(1) (IL-10/IL-19/IL-20/IL-22/IL-24/IL-26/IL-28A/IL-28B and IL-29).
IL-22 is a polypeptide of 146 amino acids with a molecular weight of 16.7 kDa(2). IL-22 structure contains six antiparallel helices(3). It is produced at a monomeric state(4) by cells from the lymphoid lineage(5) (CD4+ T cells, Th22 subpopulation, NK cells) . This cytokine acts only on non-hematopoietic stromal cells, in particular epithelial cells, keratinocytes, and hepatocytes(6).
IL-22 increases the innate immunity of tissue cells (induction of antimicrobial molecules), protects tissues from damage, and enhances their regenerations(3).
Upregulation is find in many diseases like psoriasis and Crohn's disease.
Size 96 wells
Shipping wet ice
Stability Store at +4degrees; shelf life 6months maximum after production
Specificity
Application Media It is the responsibility of the user to check the compatibility of the assay with the study matrix
Sample volume 100 µL
Tracer horseradish peroxidase HRP
Detection Limit ≤0,02 ng/mL
Standard Curve Range 0,02-2,00 ng/mL
Custom Code 3822000000
UNSPSC code 41116104

1. Sabat R IL-10 family of cytokines. 2010, Cytokine Growth Factor Rev 21:315–324

2. Xie MH, Aggarwal S, Ho WH et al. Interleukin (IL)-22, a novel human cytokine that signals through the interferon receptor-related proteins CRF2–4 and IL-22R. 2000, J Biol

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