CCL2 (pig) ELISA kit

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Cat No: A05415

Assay Kits - Elisa

Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...

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                        Territorial Availability: Available worldwide directly through Bertin or your local distributor
                    

                                            Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
                    

Synonyms:

MCP-1

chemokine (C-C motif) ligand 2

C-C motif chemokine 2

GDCF-2

HC11

HSMCR30

SCYA2

Small-inducible cytokine A2

Correlated keywords:

ELISA

Chemokine

Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
CCL2 also known as the monocyte chemoattractant protein-1 (MCP-1) is a chemokine and more particularly an C-C chemokine. Initially discovered in 1983, it is a monomeric polypeptide of 73 amino acids (13 kDa). It belongs to a family composed at least of members (MCP-1, -2, -3, and -4). CCL-2 is produced by the immun-cells (monocyte/macrophages) and non-immun cells (endothelial cells-fibroblast-myocyte). The main function of the CCL2 is the chimiotactism and more particularly the regulation of the migration and the infiltration of monocytes, memory T lymphocytes, and natural killer (NK) cells on an infection site. CCL2 is involved in many diseases like HIV-1 pathogenesis, cancer, cardiovascular disease or diabetes.

Size	96 wells
Shipping	wet ice
Specificity	refer to technical booklet
Application Media	It is the responsibility of the user to check the compatibility of the assay with the study matrix
Sample volume	100 µL
Tracer	horseradish peroxidase HRP
Detection Limit	≤3,8 pg/mL
Standard Curve Range	3,8-500,0 pg/mL
Custom Code	3822000000
UNSPSC code	41116104

Van Coillie E, Van Damme J, Opdenakker G. The MCP/eotaxin subfamily of CC chemokines. Cytokine Growth Factor (1999) Rev 10:61–86.

Yoshimura T, Yuhki N, Moore SK, Appella E, Lerman MI, Leonard EJ.Human monocyte chemoattractant protein-1 (MCP-1).Full-length cDNA cloning, expression in mitogen- stimulated blood mononuclear leukocytes, and sequence similarity to mouse competence gene. 1989b JE. FEBS Lett 244:487–49

Barna BP, Pettay J, Barnett GH, Zhou P, Iwasaki K, Estes ML. Regulation of monocyte chemoattractant protein-1 expression in adult human non-neoplastic astrocytes is sensitive to tumor necrosis factor (TNF) or antibody to the 55-kDa TNF receptor. 1994 J Neuroimmunol 50:101–107

Picker LJ, Butcher EC. Physiological and molecular mechanisms of lymphocyte homing. 1992 Annu Rev Immunol 10:561–591.

O'Connor T, Borsig L, Heikenwalder M. CCL2-CCR2 Signaling in Disease Pathogenesis. 2015 Endocr Metab Immune Disord Drug Targets.;15(2):105-18.

Technical File

MSDS