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CCL2 (pig) ELISA kit

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    CCL2 (pig) ELISA kit
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  • CCL2 (pig) ELISA kit
Cat No: A05415
Bertin Bioreagent
€395.00
Price is excluding VAT and does not include packaging neither shipping

Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...

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: 96 wells
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Territorial Availability: Available worldwide directly through Bertin or your local distributor
Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
Synonyms:
  • MCP-1
  • chemokine (C-C motif) ligand 2
  • C-C motif chemokine 2
  • GDCF-2
  • HC11
  • HSMCR30
  • SCYA2
  • Small-inducible cytokine A2
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Correlated keywords:
  • ELISA
  • EIA
  • Chemokine
Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
CCL2 also known as the monocyte chemoattractant protein-1 (MCP-1) is a chemokine and more particularly an C-C chemokine. Initially discovered in 1983, it is a monomeric polypeptide of 73 amino acids (13 kDa). It belongs to a family composed at least of members (MCP-1, -2, -3, and -4). CCL-2 is produced by the immun-cells (monocyte/macrophages) and non-immun cells (endothelial cells-fibroblast-myocyte). The main function of the CCL2 is the chimiotactism and more particularly the regulation of the migration and the infiltration of monocytes, memory T lymphocytes, and natural killer (NK) cells on an infection site. CCL2 is involved in many diseases like HIV-1 pathogenesis, cancer, cardiovascular disease or diabetes.
Size 96 wells
Shipping wet ice
Stability Store at +4degrees; shelf life 6months maximum after production
Specificity
Application Media It is the responsibility of the user to check the compatibility of the assay with the study matrix
Sample volume 100 µL
Tracer horseradish peroxidase HRP
Detection Limit ≤3,8 pg/mL
Standard Curve Range 3,8-500,0 pg/mL
Custom Code 3822000000
UNSPSC code 41116104

Van Coillie E, Van Damme J, Opdenakker G. The MCP/eotaxin subfamily of CC chemokines. Cytokine Growth Factor (1999) Rev 10:61–86.

Yoshimura T, Yuhki N, Moore SK, Appella E, Lerman MI, Leonard EJ.Human monocyte chemoattractant protein-1 (MCP-1).Full-length

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