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IL-6 (pig) ELISA kit
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Cat No: A05410
Assay Kits
- Elisa
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...
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Territorial Availability: Available worldwide directly through Bertin or your local distributor
Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
Synonyms:
- Interleukin-6
Correlated keywords:
- ELISA
- EIA
- Cytokine
- Inflammatory
- Diabetes
- Cancer
- TNF-alpha
- Interleukin-11
Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
IL-6 is a protein of 212 amino acids. It’s a pleiotropic cytokine which has an impact on the regulation of immune and nonimmune events. It is produced by the monocytes and macrophages in response to other infl ammatory cytokines like Interleukin-11 (IL-11) and the Tumor Necrosis Factor-beta (TNF-β).
IL-6 acts on immune and nonimmune cells: it helps to differentiate T-cells from B-cells but it is also vital for the development of blood cells (white cells, red cells or platelets).
Due to these target cells, IL-6 is an activator of immune system and infl ammation. It also controls the metabolism by action on adipocytes, the bone metabolism and the pain.
A dysregulation in IL-6 production induces many disorders such as autoimmune diseases, infl ammation diabetes or cancer.
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
IL-6 is a protein of 212 amino acids. It’s a pleiotropic cytokine which has an impact on the regulation of immune and nonimmune events. It is produced by the monocytes and macrophages in response to other infl ammatory cytokines like Interleukin-11 (IL-11) and the Tumor Necrosis Factor-beta (TNF-β).
IL-6 acts on immune and nonimmune cells: it helps to differentiate T-cells from B-cells but it is also vital for the development of blood cells (white cells, red cells or platelets).
Due to these target cells, IL-6 is an activator of immune system and infl ammation. It also controls the metabolism by action on adipocytes, the bone metabolism and the pain.
A dysregulation in IL-6 production induces many disorders such as autoimmune diseases, infl ammation diabetes or cancer.
| Size | 96 wells |
| Shipping | wet ice |
| Specificity | |
| Application Media | It is the responsibility of the user to check the compatibility of the assay with the study matrix |
| Sample volume | 100 µL |
| Tracer | horseradish peroxidase HRP |
| Detection Limit | ≤0,08 ng/mL |
| Standard Curve Range | 0,08-10,00 ng/mL |
| Custom Code | 3822000000 |
| UNSPSC code | 41116104 |
Toshio T., Masashi N., Tadamitsu K. IL-6 in Infl ammation, Immunity, and Disease. Cold Spring Harb Perspect Biol. 2014 Sep 4;6(10)
Kristiansen O.P. , Mandrup-Poulsen T. Interleukin-6 and Diabetes The Good, the Bad, or the Indifferent? Diabetes, 54 (2005) Supp. 2: S114-124
Scheller J., Chalaris A., Schmidt-Arras D. and al. The pro- and anti-infl ammatory properties of the cytokine interleukin-6. Biochimica et Biophysica Acta 1813 (2011) 878–888
Skurk T., Alberti-Huber C., Herde C., and al. Relationship between Adipocyte Size and Adipokine Expression and Secretion. J Clin. Endocrinol. Metab. 92 (3) (2007) 1023–1033
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