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TNF-alpha (pig) ELISA kit

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    TNF-alpha (pig) ELISA kit
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  • TNF-alpha (pig) ELISA kit
Cat No: A05411
Bertin Bioreagent
€395.00
Price is excluding VAT and does not include packaging neither shipping

Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...

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: 96 wells
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Territorial Availability: Available worldwide directly through Bertin or your local distributor
Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
Synonyms:
  • Cachectin
  • Tumor Necrosis Factor-alpha
Correlated keywords:
  • ELISA
  • EIA
  • Interleukin-1
  • Interleukin-6
  • Cytokine
  • Inflammatory
  • Chemokines
  • Swine
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Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Known as cachectin, the TNF-alpha (Tumor Necrosis Factor-alpha) is a glycoprotein of 156 amino acids produced from a precursor processed by action of a metalloprotease. Isolated for the first time in 1975 by Carswell, it’s a cytokine of the innate immunity.
Interleukin-1 or bacterial endotoxins stimulate the production of TNF-alpha by immune cells (activated macrophages, NK cells, monocytes) and non-immune cells (keratinocytes, vascular smooth muscle cells, and granulosa lutein cells).
The main function of the TNF-alpha is to act as an immunomodulator and as a mediator of infl ammatory responses in the phenomena of host resistance to infection. It allows neutrophils and macrophages recruitment to infectious sites by stimulating the production of adhesion molecules and chemokines (chemotactic cytokines) by endothelial cells. TNFalpha also acts on the hypothalamus to trigger the fever.
Over-production of TNF-alpha has been implicated in a number of pathological conditions including cachexia, septic shock, and autoimmune disorders.
Size 96 wells
Shipping wet ice
Stability Store at +4degrees; shelf life 6 months maximum after production
Specificity
Application Media It is the responsibility of the user to check the compatibility of the assay with the study matrix
Sample volume 100 µL
Tracer horseradish peroxidase HRP
Detection Limit ≤0,02 ng/mL
Standard Curve Range 0,02-2,00 ng/mL
Custom Code 3822000000
UNSPSC code 41116104

Carswell E. A., Old L. J., Kassel R. L. et al. An endotoxin-induced serum factor that causes necrosis of tumors. Proc Natl Acad Sci USA (1975) 72:3666-70

Trebichavský I., Splíchal I., Barot-Ciorbaru R. Expression of TNF-alpha in pig fetal cells stimulate

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