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- IL-10 (pig) ELISA kit
IL-10 (pig) ELISA kit
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Cat No: A05414
Assay Kits
- Elisa
€401.00
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...
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Territorial Availability: Available worldwide directly through Bertin or your local distributor
Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
Synonyms:
- Cytokine synthesis inhibitory factor
- CSIF
Correlated keywords:
- ELISA
- EIA
- Cytokine
- Tumor Necrosis Factor-alpha
- Interleukin-6
- Interleukin-1
- IL-1 beta
- Immunooncology
Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Also known as cytokine synthesis inhibitory factor (CSIF), IL-10 is a polypeptide of 157 amino acids glycosylated. The mature IL-10 is an homodimer linked by two intrachain disulfi de bridges (each sub-unit is 157 aa).
IL-10 is produced by innate and non-innate cells, it is a cytokine of the innate and adaptive immunity.
The mammalian cells known to secrete IL-10 are NK cells, cytotoxic T cells, memory Th1 and Th2 cells, macrophages monocytes, B cells, dendritic cells, hepatic stellates, keratinocytes, fetal erythroblasts.
IL-10 is a cytokine with multiple pleiotropic effects in immunoregulation and infl ammation. IL-10 is characterized like anti-infl ammatory cytokine: it represses the expression of inflammatory cytokines, such as Tumor Necrosis Factor-alpha (TNF-alpha), Interleukin-6 (IL-6) and Interleukin-1 (IL-1 beta), in macrophage. IL-10 is an immunoregulation cytokine and negative regulate Th1 responses by downmodulating antigen presenting capacity of APC.
Due its central function, a dysregulation of the production of the lL-10 is implicated in asthma or autoimmune diseases.
IL-10 is currently studied in immunooncology.
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Also known as cytokine synthesis inhibitory factor (CSIF), IL-10 is a polypeptide of 157 amino acids glycosylated. The mature IL-10 is an homodimer linked by two intrachain disulfi de bridges (each sub-unit is 157 aa).
IL-10 is produced by innate and non-innate cells, it is a cytokine of the innate and adaptive immunity.
The mammalian cells known to secrete IL-10 are NK cells, cytotoxic T cells, memory Th1 and Th2 cells, macrophages monocytes, B cells, dendritic cells, hepatic stellates, keratinocytes, fetal erythroblasts.
IL-10 is a cytokine with multiple pleiotropic effects in immunoregulation and infl ammation. IL-10 is characterized like anti-infl ammatory cytokine: it represses the expression of inflammatory cytokines, such as Tumor Necrosis Factor-alpha (TNF-alpha), Interleukin-6 (IL-6) and Interleukin-1 (IL-1 beta), in macrophage. IL-10 is an immunoregulation cytokine and negative regulate Th1 responses by downmodulating antigen presenting capacity of APC.
Due its central function, a dysregulation of the production of the lL-10 is implicated in asthma or autoimmune diseases.
IL-10 is currently studied in immunooncology.
| Size | 96 wells |
| Shipping | wet ice |
| Stability | Store at +4degrees; shelf life 6 months maximum after production |
| Specificity | |
| Application Media | It is the responsibility of the user to check the compatibility of the assay with the study matrix |
| Sample volume | 100 µL |
| Tracer | horseradish peroxidase HRP |
| Detection Limit | ≤0,16 ng/mL |
| Standard Curve Range | 0,08-10,00 ng/mL |
| Custom Code | 3822000000 |
| UNSPSC code | 41116104 |
Blancho G., Gianello P., Germana S. et al. Molecular identifi cation of porcine interleukin 10: regulation of expression in a kidney allograft model. Proc Natl Acad Sci USA 1995 Mar 28;92(7):2800-4.
Moore K.W., de Waal Malefyt R., Coffman R.L. et al. Inte
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