IL-10 (pig) ELISA kit

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Cat No: A05414

Assay Kits - Elisa

Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...

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                        Territorial Availability: Available worldwide directly through Bertin or your local distributor
                    

                                            Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
                    

Synonyms:

Cytokine synthesis inhibitory factor

CSIF

Correlated keywords:

ELISA

Cytokine

Tumor Necrosis Factor-alpha

Interleukin-6

Interleukin-1

IL-1 beta

Immunooncology

Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Also known as cytokine synthesis inhibitory factor (CSIF), IL-10 is a polypeptide of 157 amino acids glycosylated. The mature IL-10 is an homodimer linked by two intrachain disulfi de bridges (each sub-unit is 157 aa).
IL-10 is produced by innate and non-innate cells, it is a cytokine of the innate and adaptive immunity.
The mammalian cells known to secrete IL-10 are NK cells, cytotoxic T cells, memory Th1 and Th2 cells, macrophages monocytes, B cells, dendritic cells, hepatic stellates, keratinocytes, fetal erythroblasts.
IL-10 is a cytokine with multiple pleiotropic effects in immunoregulation and infl ammation. IL-10 is characterized
like anti-infl ammatory cytokine: it represses the expression of inflammatory cytokines, such as Tumor Necrosis Factor-alpha (TNF-alpha), Interleukin-6 (IL-6) and Interleukin-1 (IL-1 beta), in macrophage. IL-10 is an immunoregulation cytokine and negative regulate Th1 responses by downmodulating antigen presenting capacity of APC.
Due its central function, a dysregulation of the production of the lL-10 is implicated in asthma or autoimmune diseases.
IL-10 is currently studied in immunooncology.

Size	96 wells
Shipping	wet ice
Specificity	refer to technical booklet
Application Media	It is the responsibility of the user to check the compatibility of the assay with the study matrix
Sample volume	100 µL
Tracer	horseradish peroxidase HRP
Detection Limit	≤0,16 ng/mL
Standard Curve Range	0,08-10,00 ng/mL
Custom Code	3822000000
UNSPSC code	41116104

Blancho G., Gianello P., Germana S. et al. Molecular identifi cation of porcine interleukin 10: regulation of expression in a kidney allograft model. Proc Natl Acad Sci USA 1995 Mar 28;92(7):2800-4.

Moore K.W., de Waal Malefyt R., Coffman R.L. et al. Interleukin-10 and the interleukin-10 receptor. Annu Rev Immunol. 2001;19:683-765.

Windsor W.T., Syto R., Tsarbopoulos A. et al. Disulfi de bond assignments and secondary structure analysis of human and murine interleukin 10. Biochemistry. 1993 Aug 31;32(34):8807-15.

Mehrotra P.T., Donnelly R.P., Wong S. et al. Production of IL-10 by human natural killer cells stimulated with IL-2 and/or IL-12. J Immunol. 1998 Mar 15;160(6):2637-44.

Yssel H., De Waal Malefyt R., Roncarolo M.G. et al. IL-10 is produced by subsets of human CD4+ T cell clones and peripheral blood T cells.J Immunol. 1992 Oct 1;149(7):2378-84.

Grewe M., Gyufko K., Krutmann J. Interleukin-10 production by cultured human keratinocytes: regulation by ultraviolet B and ultraviolet A1 radiation. J Invest Dermatol. 1995 Jan;104(1):3-6.

Sennikov S.V., Krysov S.V., Silkov A.N. Production of IL-10, TNF-alpha, IFN-gamma, TGF-beta by different populations of erythroid cells derived from human embryonal liver. Cytokine. 2002 Feb 21;17(4):221-5.

Williams L.M., Ricchetti G., Sarma U. et al. Interleukin-10 suppression of myeloid cell activation - acontinuing puzzle. Immunology 2004 Nov;113(3):281-92

Hu X., Chen J., Wang L. et al Crosstalk among Jak-STAT, Toll-like receptor, and ITAMdependent pathways in macrophage activation. Journal of leukocyte biology 2007 Aug;82(2):237-43

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