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IL-8 (pig) ELISA kit

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    IL-8 (pig) ELISA kit
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  • IL-8 (pig) ELISA kit
Cat No: A05421
Bertin Bioreagent
€395.00
Price is excluding VAT and does not include packaging neither shipping

Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...

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: 96 wells
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Territorial Availability: Available worldwide directly through Bertin or your local distributor
Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
Synonyms:
  • Interleukin-8
  • CXCL8
  • GCP-1
  • GCP1
  • LECT
  • LUCT
  • LYNAP
  • MDNCF
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Correlated keywords:
  • ELISA
  • EIA
  • Chemokine
  • CXCR1
  • CXCR2
  • angiogenic
  • inflammation
  • Interleukin-8
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Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
IL-8 is a polypeptide of 72-77 amino acids(1), known also as CXCL8, GCP-1 and NAP-1. Member of the CXC chemokine family, it can adopt a monomeric or homodimeric conformation containing a triple-stranded anti-parallel beta-sheet arranged in a Greek key and a long C-terminal helix(2). IL-8 could also form a heterodimer with PF4 activity(3). The receptors of IL-8 are CXCR1 and CXCR2(4).
IL-8 is mainly produced by macrophages(5). It acts as an angiogenic factor in human microvascular endothelial cells(6). The main role of IL-8 in inflammation is the recruitment of neutrophils(7), although it is also responsible for the chemotactic migration and activation of monocytes, lymphocytes, basophils, and eosinophils at sites of inflammation(8).
Size 96 wells
Shipping wet ice
Stability Store at +4degrees; shelf life 6months maximum after production
Specificity
Application Media It is the responsibility of the user to check the compatibility of the assay with the study matrix
Sample volume 100 µL
Tracer horseradish peroxidase HRP
Detection Limit ≤0,01 ng/mL
Standard Curve Range 0,01-1,00 ng/mL
Custom Code 3822000000
UNSPSC code 41116104

1. Brat DJ, Bellail AC, Van Meir EG The role of interleukin-8 and its receptors in gliomagenesis and tumoral angiogenesis 2005, Neuro-oncology. 7 (2): 122–133

2. G. Marius CloreSj, Ettore Appellaq, Masaki Yamadall et al. Determination of the Secondary Str