IL-8 (pig) ELISA kit

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Cat No: A05421

€395.00

Price is excluding VAT and does not include packaging neither shipping

Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...

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                        Territorial Availability: Available worldwide directly through Bertin or your local distributor
                    

                                            Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
                    

Synonyms:

Interleukin-8

CXCL8

GCP-1

GCP1

LECT

LUCT

LYNAP

MDNCF

MONAP

NAP-1

NAP1

T cell chemotactic factor

beta-thromboglobulin-like protein

chemokine (C-X-C motif) ligand 8

chemoctakin

granulocyte chemotactic

Correlated keywords:

ELISA

Chemokine

CXCR1

CXCR2

angiogenic

inflammation

Interleukin-8

chemotactic

monocytes

lymphocytes

basophils

eosinophils

CXCL8

GCP-1

GCP1

LECT

LUCT

LYNAP

MDNCF

MONAP

NAP-1

NAP1

T cell chemotactic factor

beta-thromboglobulin-like protein

chemo

Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
IL-8 is a polypeptide of 72-77 amino acids(1), known also as CXCL8, GCP-1 and NAP-1. Member of the CXC chemokine family, it can adopt a monomeric or homodimeric conformation containing a triple-stranded anti-parallel beta-sheet arranged in a Greek key and a long C-terminal helix(2). IL-8 could also form a heterodimer with PF4 activity(3). The receptors of IL-8 are CXCR1 and CXCR2(4).
IL-8 is mainly produced by macrophages(5). It acts as an angiogenic factor in human microvascular endothelial cells(6). The main role of IL-8 in inflammation is the recruitment of neutrophils(7), although it is also responsible for the chemotactic migration and activation of monocytes, lymphocytes, basophils, and eosinophils at sites of inflammation(8).

Size	96 wells
Shipping	wet ice
Stability	Store at +4degrees; shelf life 6months maximum after production
Specificity	refer to technical booklet
Application Media	It is the responsibility of the user to check the compatibility of the assay with the study matrix
Sample volume	100 µL
Tracer	horseradish peroxidase HRP
Detection Limit	≤0,01 ng/mL
Standard Curve Range	0,01-1,00 ng/mL
Custom Code	3822000000
UNSPSC code	41116104

1. Brat DJ, Bellail AC, Van Meir EG The role of interleukin-8 and its receptors in gliomagenesis and tumoral angiogenesis 2005, Neuro-oncology. 7 (2): 122–133

2. G. Marius CloreSj, Ettore Appellaq, Masaki Yamadall et al. Determination of the Secondary Str

Technical File

MSDS