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IL-8 (pig) ELISA kit
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Cat No: A05421
Assay Kits
- Elisa
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...
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Territorial Availability: Available worldwide directly through Bertin or your local distributor
Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
Correlated keywords:
- ELISA
- EIA
- Chemokine
- CXCR1
- CXCR2
- angiogenic
- inflammation
- Interleukin-8
Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
IL-8 is a polypeptide of 72-77 amino acids(1), known also as CXCL8, GCP-1 and NAP-1. Member of the CXC chemokine family, it can adopt a monomeric or homodimeric conformation containing a triple-stranded anti-parallel beta-sheet arranged in a Greek key and a long C-terminal helix(2). IL-8 could also form a heterodimer with PF4 activity(3). The receptors of IL-8 are CXCR1 and CXCR2(4).
IL-8 is mainly produced by macrophages(5). It acts as an angiogenic factor in human microvascular endothelial cells(6). The main role of IL-8 in inflammation is the recruitment of neutrophils(7), although it is also responsible for the chemotactic migration and activation of monocytes, lymphocytes, basophils, and eosinophils at sites of inflammation(8).
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
IL-8 is a polypeptide of 72-77 amino acids(1), known also as CXCL8, GCP-1 and NAP-1. Member of the CXC chemokine family, it can adopt a monomeric or homodimeric conformation containing a triple-stranded anti-parallel beta-sheet arranged in a Greek key and a long C-terminal helix(2). IL-8 could also form a heterodimer with PF4 activity(3). The receptors of IL-8 are CXCR1 and CXCR2(4).
IL-8 is mainly produced by macrophages(5). It acts as an angiogenic factor in human microvascular endothelial cells(6). The main role of IL-8 in inflammation is the recruitment of neutrophils(7), although it is also responsible for the chemotactic migration and activation of monocytes, lymphocytes, basophils, and eosinophils at sites of inflammation(8).
| Size | 96 wells |
| Shipping | wet ice |
| Specificity | |
| Application Media | It is the responsibility of the user to check the compatibility of the assay with the study matrix |
| Sample volume | 100 µL |
| Tracer | horseradish peroxidase HRP |
| Detection Limit | ≤0,01 ng/mL |
| Standard Curve Range | 0,01-1,00 ng/mL |
| Custom Code | 3822000000 |
| UNSPSC code | 41116104 |
1. Brat DJ, Bellail AC, Van Meir EG The role of interleukin-8 and its receptors in gliomagenesis and tumoral angiogenesis 2005, Neuro-oncology. 7 (2): 122–133
2. G. Marius CloreSj, Ettore Appellaq, Masaki Yamadall et al. Determination of the Secondary Structure of Interleukin-8 by Nuclear Magnetic Resonance Spectroscopy 1989, The Journal Of Biologica Chemistry 264, No. 32, Issue of November 15, pp. 18907-18911
3. Irina V. Nesmelova, Yuk Sham et al. Platelet Factor 4 and Interleukin-8 CXC Chemokine Heterodimer Formation Modulates Function at the Quaternary Structural Level 2005, J. Biol. Chem. 280:4948-4958
4. James Lee, Richard Horuk, Glenn C. Rice et al. Characterization of Two High Affinity Human Interleukin-8 Receptors 1992, J. Biol. Chem. 267:16283-7
5. Mark D. Turner, Belinda Nedjai, Tara Hurst, Daniel J. Pennington Cytokines and chemokines: At the crossroads of cell signalling and inflammatory disease 2004, Biochimica et Biophysica Acta 1843 2563–2582
6. G J. Heidemann, H. Ogawa, M.B. Dwinell et al. Angiogenic effects of interleukin 8 (CXCL8) in human intestinal microvascular endothelial cells are mediated by CXCR2 2003, J. Biol. Chem. 278 8508–8515.
7. M.E. Hammond, G.R. Lapointe, P.H. Feucht et al. IL-8 induces neutrophil chemotaxis predominantly via type I IL-8 receptors 1995, J. Immunol. 155 1428–1433.
8. E.J. Miller, A.B. Cohen, S. Nagao et al. Elevated levels of NAP-1/interleukin-8 are present in the airspaces of patientswith the adult respiratory distress syndrome and are associated with increased mortality 1992, Am. Rev. Respir. Dis.146 427-432
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