IL-28B (pig) ELISA kit

Assay Kits - ELISA

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Cat No: A05425

Assay Kits - Elisa

Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...

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                        Territorial Availability: Available worldwide directly through Bertin or your local distributor
                    

                                            Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
                    

Synonyms:

Interleukin-28B

Correlated keywords:

ELISA

Chemokin

epithelial cells

keratinocytes

hepatocyte

antiviral

Interleukin-28B

Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Interleukin 28B (IL-28B), recently renamed interferon- λ3 (IFNλ3), is one of the 9 members of the IL-10 family(1) (IL-10/IL-19/IL-20/IL-22/IL-24/IL-26/IL-28A/IL-28B and IL-29). It has been classified as type III interferon(2).
IL-28B is a polypeptide of 170 amino acids with a molecular weight of 19.6 kDa. IL-28B structure contains six antiparallel helices(3)
This cytokine is produced at a monomeric state(4) by plasmacytoid dendritic cells after virus infection or dsRNA. Moreover its production is enhanced by Interferon-α.
The main function of IL28B is antiviral action by the modulation of the expression of MHC (Type I/II) in dentritic cells or by the modulation of CCR7 receptor mediating dentritic cells migration.

Size	96 wells
Shipping	wet ice
Specificity	refer to technical booklet
Application Media	It is the responsibility of the user to check the compatibility of the assay with the study matrix
Sample volume	100 µL
Tracer	horseradish peroxidase HRP
Detection Limit	≤0,02 ng/mL
Standard Curve Range	0,02-2,00 ng/mL
Custom Code	3822000000
UNSPSC code	41116104

1. Sabat R IL-10 family of cytokines 2010, Cytokine Growth Factor Rev 21 : 315–324

2. Zdanov A (2010) Structural analysis of cytokines comprising the IL-10 family. 2010, Cytokine Growth Factor Rev 21 : 325–330

3. Robert Sabat, Ellen Witte, Katrin Witte, and Kerstin Wolk IL-22 and IL-17: An Overview 2013, IL-17, IL-22 and Their Producing Cells: Role in Inflammation and Autoimmunity, 2nd ed., pp.11-35

4. Zdanov A Structural analysis of cytokines comprising the IL-10 family. 2010, Cytokine Growth Factor Rev 21 :325–330

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