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- Unacylated Ghrelin (human) Easy Sampling ELISA kit
Unacylated Ghrelin (human) Easy Sampling ELISA kit
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Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...
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Territorial Availability: Available worldwide directly through Bertin or your local distributor
Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
Synonyms:
- Unacylated Ghrelin (human) EIA kit
- Des-octanoyl Ghrelin
Correlated keywords:
- ELISA
- spi-bio
- acylated
- immunoassays
- obesity
- growth
- hormones
- homeostasis
Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Bertin Bioreagent offers a totally new set of assays to measure Acylated & Unacylated Ghrelin, whatever the collection procedure.
Those assays are called Easy Sampling EIA kit because any blood sample collected can be used on any kind of protease inhibitors with or without acidification. From early discovery programs using our 384 wells plate format to select your GOAT inhibitor, to the pre-clinical or clinical stage, Bertin Bioreagent has a solution.
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Bertin Bioreagent offers a totally new set of assays to measure Acylated & Unacylated Ghrelin, whatever the collection procedure.
Those assays are called Easy Sampling EIA kit because any blood sample collected can be used on any kind of protease inhibitors with or without acidification. From early discovery programs using our 384 wells plate format to select your GOAT inhibitor, to the pre-clinical or clinical stage, Bertin Bioreagent has a solution.
| Size | 96 wells |
| Shipping | wet ice |
| Specificity | |
| Application Media | Plasma, buffer|Blood collection on inhibitor then dilution prior assay |
| Tracer | AcetylCholinesterase AChE |
| Detection Limit | refer to technical booklet |
| Standard Curve Range | 2-250 pg/mL |
| Custom Code | 3822.00 |
| UNSPSC code | 41116104 |
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PRODUCT EXPLANATION: ACETYLCHOLINESTERASE MARKER IN ENZYME-IMMUNOASSAYS
Grassi J., Pradelles P. Compounds labelled by the acetylcholinesterase of Electrophorus Electricus. Its preparation process and its use as a tracer or marquer in enzymo-immunological determinations. United States patent, N° 1,047,330. September 10, 1991
Grassi J., Pradelles P. The use of Acetylcholinesterase as a Universal marker in Enzyme-Immunoassays. Proceedings of the Third International Meeting on Cholinesterases, American Chemical Society (1991)
Pradelles P., Grassi J., Maclouf J.Enzyme Immunoassays of Eicosanoids Using Acetylcholinesterase. Methods in Enzymology (1990), vol. 187, 24-34
PRODUCT EXPLANATION: GHRELIN
Kojima M, Kangawa K. Ghrelin: structure and function. Physiol. Rev ( 2005), 85:495-522
Bluet-Pajot MT, Tolle V, Zizzari P, Tomasetto C, Grouselle D, Epelbaum J. Ghrelin: A striking example of neuroendocrine peptide pleiotropy. Med Sci (Paris), August 1, 2005, 21 (8-9): 715-21
Müller TD, Nogueiras R, Andermann ML et al. Ghrelin. Mol Metab. 2015 Mar 21;4(6):437-60
de Faria Barros A et al. Is there association between acyl-ghrelin and inflammation in hemodialysis patients? J Bras Nefrol. (2013) 35(2):120-126.
Grousselle D et al. Variations des peptides dérivés de la préproghréline au cours du repas dans l’anorexie mentale restrictive. Poster GIR-AFDAS-TCA2014
Delhanty P et al. Des-acyl ghrelin analogs prevent high-fat-diet-induced dysregulation of glucose homeostasis. FASEB J. (2013) 27(4):1690-1700
Costantini, V et al. GSK1614343, a Novel Ghrelin Receptor Antagonist, Produces an Unexpected Increase of Food Intake and Body Weight in Rodents and Dogs. Neuroendocrinology (2011);94:158–168
Porporato E, Filigheddu N et al. Acylated and unAcylated Ghrelin impair skeletal muscle atrophy in mice. J. Clinical Invest (2013) 123(2): 611-622
Sentissi O, Epelbaum J, Olié JP, Poirier MF. Leptin and Ghrelin Levels in Patients With Schizophrenia During Different Antipsychotics Treatment: A Review. Schizophrenia Bulletin (2008) 34(6), 1189–1199
PRODUCT EXPLANATION: KIT VALIDATION
Valentin MA, Ma S, Zhao A, Legay F, Avrameas A. Validation of immunoassay for protein biomarkers: Bioanalytical study plan implementation to support pre-clinical and clinical studies. J Pharm Biomed Anal. (2011) 55(5) : 869-877
European Medicines Agency. Guideline on bioanalytical method validation, 21 July 2011.
CITATIONS OF SPI-BIO HUMAN GHRELIN KITS
Beauloye V, Diene G, Kuppens R et al. High unacylated ghrelin levels support the concept of anorexia in infants with prader-willi syndrome. Orphanet J Rare Dis. 2016 May 4;11(1):56. doi: 10.1186/s13023-016-0440-0
Kuppens RJ, Diène G, Bakker NE et al. Elevated ratio of acylated to unacylated ghrelin in children and young adults with Prader-Willi syndrome. Endocrine. 2015 Dec;50(3):633-42. doi: 10.1007/s12020-015-0614-x. Epub 2015 May 20
Vestergaard ET, Jessen N, Moller N, Jorgensen JO. Acyl Ghrelin Induces Insulin Resistance Independently of GH, Cortisol, and Free Fatty Acids. Sci Rep. 2017 Feb 15; 7:42706. doi: 10.1038/srep42706
van Adrichem RC, van der Lely AJ, Huisman M et al. Plasma acylated and plasma unacylated ghrelin: useful new biomarkers in patients with neuroendocrine tumors? Endocr Connect. 2016 Jul;5(4):143-51. doi: 10.1530/EC-16-0021. Epub 2016 May 23
Lauritzen ES, Voss T, Kampmann U et al. Circulating acylghrelin levels are suppressed by insulin and increase in response to hypoglycemia in healthy adult volunteers. Eur J Endocrinol. 2015 Apr;172(4):357-62. doi: 10.1530/EJE-14-0880. Epub 2015 Jan 19
Kanat BH, Ayten R, Aydın S et al. Significance of appetite hormone ghrelin and obestatin levels in the assessment of the severity of acute pancreatitis. Turk J Gastroenterol. 2014 Jun;25(3):309-13. doi: 10.5152/tjg.2014.4081
Delhanty P. et al. The Acylated (AG) to Unacylated (UAG) Ghrelin Ratio in Esterase Inhibitor-Treated Blood Is Higher Than Previously Described. Clin Endocrinol (Oxf). 2015 Jan;82(1):142-6. doi: 10.1111/cen.12489. Epub 2014 Jun 2
Homann D. et al. Hyperleptinemia independent of body adiposity in women with fibromyalgia. Rheumatol Int (2014) DOI 10.1007/s00296-014-2988-0
Wasse L. et al. The influence of vigorous running and cycling exercise on hunger perceptions and plasma acylated ghrelin concentrations in lean young men. Appl. Physiol. Nutr. Metab.(2013) dx.doi.org/10.1139/apnm-2012-0154
Gungor S. et al. Ghrelins, obestatin, nesfatin-1 and leptin levels in pregnant women with 2 and without hyperemesis gravidarum. Clin Biochem. (2013) DOI: 10.1016/j.clinbiochem.2013.01.015
Broom D. et al. Influence of resistance and aerobic exercise on hunger, circulating levels of acylated ghrelin, and peptide YY in healthy males. Am J Physiol Regulatory Integrative Comp Physiol (2009) doi:10.1152/ajpregu.90706.2008
Broom D. et al. Exercise-induced suppression of acylated ghrelin in humans. J Appl Physiol (2007) doi:10.1152/japplphysiol.00759.2006
Lee Y. et al. Lupin-enriched bread increases satiety and reduces energy intake acutely. Am J Clin Nutr (2006) doi: 10.3945/ ajcn.2008.26708
Cederberg H. et al. Unacylated ghrelin is associated with changes in body composition and body fat distribution during long-term exercise intervention. European Journal of Endocrinology (2011) DOI: 10.1530/EJE-11-0334
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