Adiponectin High Sensitivity (human) ELISA kit

Assay Kits - ELISA

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Cat No: A05186

Assay Kits - Elisa

€458.00

Price is excluding VAT and does not include packaging neither shipping

Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...

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                        Territorial Availability: Available worldwide directly through Bertin or your local distributor
                    

                                            Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
                    

Synonyms:

Adiponectin (HS) (human) EIA kit

Correlated keywords:

calcitonin

peptide

measures

enzyme

immunoassays

ELISA

Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Adiponectin, also referred to as Acrp30, AdipoQ and GBP-28, is an 244 aminoacid protein, which is physiologically active, specifically and highly expressed in adipose cells (adipokine). Adiponectin forms homotrimers, which are the building blocks for higher order complexes found circulating in serum. Paradoxically, adipose tissue-expressed adiponectin levels are inversely related to the degree of adiposity. A reduction in adiponectin serum levels is accompanied by insulin resistance states, such as obesity and type II diabetes mellitus. Adiponectin has been shown to increase insulin sensitivity and decrease plasma glucose by increasing tissue fat oxidation. It inhibits the inflammatory processes of atherosclerosis suppressing the expression of adhesion and cytokine molecules in vascular endothelial cells and macrophages, respectively.

Size	96 wells
Shipping	Wet ice
Stability	Store at +4degrees; shelf life 6 months maximum after production
Specificity	Human Leptin <0.1 % Human Resistin <0.1 % Human Leptin Reactor <0.1 % Sheep, Goat, Horse, Cow Pig and Rabbit Adiponectin <0.1 % Monkey Serum equivalent to 15-19 µg/mL
Application Media	Human Serum, Plasma, Breast Milk, Urine and Tissue Culture Supernatant\|1/300 dilution for serum and plasma, 1/3 for breast milk and urine prior assay
Sample volume	5 µL
Tracer	HRP
Detection Limit	0.5 ng/mL
Standard Curve Range	5-150 ng/mL for serum and plasma
Custom Code	3822000000
UNSPSC code	41116104

Scherer PE, Williams S, Fogliano M, Baldini G, Lodish HF. A novel serum protein similar to C1q, produced exclusively in adipocytes. J Biol Chem. 1995, 270, 26746-26749.

Maeda K, Okubo K, Shimomura I, Funahashi T, Matsuzawa Y, Matsubara K. cDNA cloning and

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