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Leptin (mouse, rat) ELISA kit
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Cat No: A05176
Assay Kits
- Elisa
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...
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Territorial Availability: Available worldwide directly through Bertin or your local distributor
Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
Synonyms:
- Leptin (mouse/rat) EIA kit
Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Leptin, the product of the ob (obese) gene, is produced mainly in the adipose tissue, and is considered to play an important role in appetite control, fat metabolism and body weight regulation. The primary effect of leptin appears to be mediated by leptin receptors expressed mainly in the hypothalamus. In humans, leptin levels correlate with body mass index (BMI) and percentage body fat, and are elevated even in obese individuals. Leptin has a dual action; it decreases the appetite and increases energy consumption. Leptin is secreted in circadian fashion with nocturnal rise in both lean and obese patients. Mutations of the ob gene resulting in leptin deficiency are the cause of obesity in the ob/ob mice suggesting that endogenous leptin can normalize body weight. In contrast, human obese subjects may have high levels of leptin, indicating a mechanism of leptin resistance.
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Leptin, the product of the ob (obese) gene, is produced mainly in the adipose tissue, and is considered to play an important role in appetite control, fat metabolism and body weight regulation. The primary effect of leptin appears to be mediated by leptin receptors expressed mainly in the hypothalamus. In humans, leptin levels correlate with body mass index (BMI) and percentage body fat, and are elevated even in obese individuals. Leptin has a dual action; it decreases the appetite and increases energy consumption. Leptin is secreted in circadian fashion with nocturnal rise in both lean and obese patients. Mutations of the ob gene resulting in leptin deficiency are the cause of obesity in the ob/ob mice suggesting that endogenous leptin can normalize body weight. In contrast, human obese subjects may have high levels of leptin, indicating a mechanism of leptin resistance.
| Size | 96 wells |
| Shipping | Wet ice |
| Specificity | |
| Application Media | Mouse and rat serum, plasma and culture supernatant|1/20 to 1/10 dilution prior assay |
| Sample volume | 15 µL |
| Tracer | HRP |
| Detection Limit | 50 pg/mL |
| Standard Curve Range | 63-4000 pg/mL |
| Custom Code | 3822000000 |
| UNSPSC code | 41116104 |
PRODUCT EXPLANATION: LEPTIN
Gruver AL., Sempowski GD. Cytokines, leptin, and stress-induced thymic atrophy. J Leukoc Biol. (2008) May
Cherhab FF., Mounzih K., Lu R. et al. Early onset of reproductive function in normal mice treated with Leptin. Science 275, 88 (1997)
Clement K., Vaisse C., Lahlou . et al. A mutation in the human leptin receptor gene causes obesity and pituitary dysfunction. Nature 329, 398 (1998)
Considine R.V., Sinha M.K., Heiman M.L. et al. Serum immunoreactive leptin concentrations in normal-weight and obese humans. N. Engl. J. Med. 334, 292-295 (1996)
Friedman JM., Halaas JL. Leptin and regulation of body weight in mammals. Nature 395, 763 (1998)
Halaas J.L., Gajiwala K.S., Maffei M.. et al. Weight-reducing effects of the plasma protein encoded by the obese gene. Science 269, 543-546 (1995)
Montague CT., Faroozi IS., Witehead JP. et al. Congenital leptin defi ciency defi ciency is associated with serve early-onset obesity in humans. Nature 387, 903 (1997)
Pelleymounter M.A., Cullen M.J., Baker M.B. et al. Effects of the obese gene product on body weight regulation in ob/ob mice. Science 269, 540-543 (1995)
Zhang Y., Proenca R., Maffei M.. et al. Positional cloning of the mouse obese gene and its human homologue. Nature 372, 425-432 (1994)
PRODUCT EXPLANATION: KIT DEVELOPMENT
Valentin MA, Ma S, Zhao A, Legay F, Avrameas A. Validation of immunoassay for protein biomarkers: Bioanalytical study plan implementation to support pre-clinical and clinical studies. J Pharm Biomed Anal. (2011) 55(5): 869-877
European Medicines Agency. Guideline on bioanalytical method validation, 21 July 2011
CITATIONS OF SPI-BIO LEPTIN KIT
Mashmoul M, Azlan A, Mohtarrudin N et al, Saffron Extract and Crocin Reduced Biomarkers Associated with Obesity in Rats Fed a High-Fat Diet. Mal J Nutr 23(1) : 117 - 127, 2017
Noaman I.M, The Effect of Serotonin on Leptin and Grelin Hormones Concentrations in Female Rats. Kufa Journal For Veterinary Medical Sciences Vol. (6) No. (2) 2015
Hoile SP, Grenfell LM, Hanson MA et al, Fat and carbohydrate intake over three generations modify growth, metabolism and cardiovascular phenotype in female mice in an age-related manner. PLoS One. 2015 Aug 12 ;10(8) : e0134664
Lefils J, Géloën A, Vidal H et al, Dietary DHA : time course of tissue uptake and effects on cytokine secretion in mice. Br J Nutr. 2010 Nov ;104(9) :1304-12
Aminzadeh MA, Pahl MV, Barton CH et al, Human uraemic plasma stimulates release of leptin and uptake of tumour necrosis factor-alpha in visceral adipocytes. Nephrol Dial Transplant. 2009 Dec ;24(12) :3626-31
Pye KM1, Wakefield AP, Aukema HM et al, A high mixed protein diet reduces body fat without altering the mechanical properties of bone in female rats. J Nutr. 2009 Nov ;139(11) :2099-105
Terao S, Yilmaz G, Stokes KY et al, Inflammatory and injury responses to ischemic stroke in obese mice. Stroke. 2008 Mar ;39(3) :943-50
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