Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...
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Size | 96 wells |
Shipping | wet ice |
Specificity | |
Application Media | Plasma, buffer|Blood collection on inhibitor then dilution prior assay |
Tracer | AcetylCholinesterase AChE |
Detection Limit | refer to technical booklet |
Standard Curve Range | 2-250 pg/mL |
Custom Code | 3822.00 |
UNSPSC code | 41116104 |
PRODUCT EXPLANATION: ACETYLCHOLINESTERASE MARKER IN ENZYME-IMMUNOASSAYS
Grassi J., Pradelles P. Compounds labelled by the acetylcholinesterase of Electrophorus Electricus. Its preparation process and its use as a tracer or marquer in enzymo-immunological determinations. United States patent, N° 1,047,330. September 10, 1991
Grassi J., Pradelles P. The use of Acetylcholinesterase as a Universal marker in Enzyme-Immunoassays. Proceedings of the Third International Meeting on Cholinesterases, American Chemical Society (1991)
. Pradelles P., Grassi J., Maclouf J. Enzyme Immunoassays of Eicosanoids Using Acetylcholinesterase. Methods in Enzymology (1990), vol. 187, 24-34
PRODUCT EXPLANATION: GHRELIN
Kojima M, Kangawa K. Ghrelin: structure and function. Physiol. Rev ( 2005), 85:495-522
Müller TD, Nogueiras R, Andermann ML et al. Ghrelin. Mol Metab. 2015 Mar 21;4(6):437-60
Bluet-Pajot MT, Tolle V, Zizzari P, Tomasetto C, Grouselle D, Epelbaum J. Ghrelin: A striking example of neuroendocrine peptide pleiotropy. Med Sci (Paris), August 1, 2005, 21 (8-9): 715-21
de Faria Barros A et al. Is there association between acyl-ghrelin and inflammation in hemodialysis patients? J Bras Nefrol. (2013) 35(2):120-126
Grousselle D et al. Variations des peptides dérivés de la préproghréline au cours du repas dans l’anorexie mentale restrictive. Poster GIR-AFDAS-TCA2014
Delhanty P et al. Des-acyl ghrelin analogs prevent high-fat-diet-induced dysregulation of glucose homeostasis. FASEB J. (2013) 27(4):1690-1700
Costantini, V et al. GSK1614343, a Novel Ghrelin Receptor Antagonist, Produces an Unexpected Increase of Food Intake and Body Weight in Rodents and Dogs. Neuroendocrinology (2011);94:158–168
Porporato E, Filigheddu N et al. Acylated and unAcylated Ghrelin impair skeletal muscle atrophy in mice. J. Clinical Invest (2013) 123(2): 611-622
Sentissi O, Epelbaum J, Olié JP, Poirier MF. Leptin and Ghrelin Levels in Patients With Schizophrenia During Different Antipsychotics Treatment: A Review. Schizophrenia Bulletin (2008) 34(6), 1189–1199
PRODUCT EXPLANATION: KIT VALIDATION
Valentin MA, Ma S, Zhao A, Legay F, Avrameas A. Validation of immunoassay for protein biomarkers: Bioanalytical study plan implementation to support pre-clinical and clinical studies. J Pharm Biomed Anal. (2011) 55(5) : 869-877
European Medicines Agency. Guideline on bioanalytical method validation, 21 July 2011
CITATIONS OF GHRELIN KITS
Hassouna, Grouselle D, Chiappetta G et al. Combination of Selective Immunoassays and Mass Spectrometry to Characterize Preproghrelin-Derived Peptides in Mouse Tissues. Front Neurosci. 2017 Apr 20;11:211
Beauloye V, Diene G, Kuppens R et al. High unacylated ghrelin levels support the concept of anorexia in infants with prader-willi syndrome. Orphanet J Rare Dis. 2016 May 4;11(1):56
Erden I, Uçak H, Demir B et al. Serum ghrelin levels in patients with Behcet's disease.Postepy Dermatol Alergol. 2016 Dec;33(6):450-456
Gagnon J, Anini Y. Insulin and Norepinephrine Regulate Ghrelin Secretion from a Rat Primary Stomach Cell Culture. Endocrinology (2012) doi 10.1210/en.2012-1040
Lin Li R, Sherbet D et al. Profound Hypoglycemia in Starved, Ghrelin-deficient Mice Is Caused by Decreased Gluconeogenesis and Reversed by Lactate or Fatty Acids. JBC (2012)
Lu X et al. Postprandial inhibition of gastric ghrelin secretion by long-chain fatty acid through GPR120 in isolated gastric ghrelin cells and mice. Am J Physiol Gastrointest Liver Physiol (2012)doi:10.1152/ajpgi.00541.2011
Robert F et al. Targeting Protein Synthesis in a Myc/mTOR-Driven Model of Anorexia-Cachexia Syndrome Delays Its Onset and Prolongs Survival. Cancer Res (2012) doi:10.1158/0008-5472.CAN-11-2739
Teubner B et al. Inhibition of ghrelin O-acyltransferase attenuates food deprivation-induced increases in ingestive behavior. Hormones and Behavior (2013)