CGRP (rat) ELISA kit

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Cat No: A05482

Assay Kits - Elisa

Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...

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Scientific Literature
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                        Territorial Availability: Available worldwide directly through Bertin or your local distributor
                    

                                            Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
                    

Synonyms:

Calcitonin Gene-Related Peptide (rat) ELISA kit

Correlated keywords:

calcitonin

peptide

measures

enzyme

immunoassays

ELISA

neurochemistry

neuroscience

vasodilator

pain

migraine

spi-bio

spibio

A05482

CGRP

calcitonin receptor-like receptor (CLR or CALCRL)

receptor activity modifying protein (RAMP)

589001

Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Calcitonin Gene Related Peptide (CGRP) is a potent vasodilator, and also elicits a number of other biological effects. Average plasma levels of CGRP have been reported to be from 0.8 pmol/L to 71 pmol/l (3 pg/mL to 269 pg/mL) in normal subjects. Increases in circulating CGRP levels have been noticed during hemodialysis, pregnancy, exacerbation of asthma, and in cases of medullary thyroid carcinoma.

Size	96 wells
Shipping	wet ice
Specificity	Rat CGRP-I/II: 100% Rat CGRP-α/β: 100% Human CGRP-I/II: 83% Human CGRP-α/β: 100% CGRP (8-37): <0.01% Amylin: <0.01% Calcitonin: <0.01% Substance P: <0;01%
Application Media	Rat blood, plasma serum, culture supernatant, nervous tissues, cerebrospinal fluid\|Plasma & serum: no extraction requiredif free plasma or serum used dor standard curve.\|Cerebrospinal fluid: 1/20 dilution prior assay.\|Other nervous tissue: extraction with
Sample volume	100 µl
Tracer	AcetylCholinesterase AChE
Detection Limit	0.7 pg/mL
Standard Curve Range	4-500 pg/mL
Custom Code	3822.00
UNSPSC code	41116104

PRODUCT EXPLANATION: ACETYLCHOLINESTERASE MARKER IN ENZYME-IMMUNOASSAYS

Grassi J., Pradelles P. Compounds labelled by the acetylcholinesterase of Electrophorus Electricus. Its preparation process and its use as a tracer or marquer in enzymo-immunological determinations. United States patent, N° 1,047,330. September 10, 1991

Grassi J., Pradelles P. The use of Acetylcholinesterase as a Universal marker in Enzyme-Immunoassays. Proceedings of the Third International Meeting on Cholinesterases, American Chemical Society (1991)

Pradelles P., Grassi J., Maclouf J. Enzyme Immunoassays of Eicosanoids Using Acetylcholinesterase. Methods in Enzymology (1990), vol. 187, 24-34

PRODUCT EXPLANATION: CGRP

Russell FA, King R, Smillie SJ et al. Calcitonin gene-related peptide: physiology and pathophysiology. Physiol Rev. 2014 Oct;94(4):1099-142

Schou WS, Ashina S, Amin FM et al. Calcitonin gene-related peptide and pain: a systematic review. J Headache Pain. 2017 Dec;18(1):34

PRODUCT EXPLANATION: CGRP KIT DEVELOPMENT

Frobert Y., Nevers M.-C., Amadesi S., Volland H., Brune P., Geppetti P., Grassi J. & Créminon C. A sensitive sandwich enzyme immunoassay for calcitonin gene-related peptide (CGRP): characterization and application. Peptide 20 (1999) 275-284

Valentin MA, Ma S, Zhao A, Legay F, Avrameas A. Validation of immunoassay for protein biomarkers: Bioanalytical study plan implementation to support pre-clinical and clinical studies. J Pharm Biomed Anal. (2011) 55(5) : 869-877

European Medicines Agency. Guideline on bioanalytical method validation, 21 July 2011

PRODUCT CITATIONS

Boros K, Jancsó G, Dux M et al. Multiple impairments of cutaneous nociceptor function induced by cardiotoxic doses of Adriamycin in the rat. Naunyn Schmiedebergs Arch Pharmacol. 2016 Sep;389(9):1009-20

Dux M, Will C, Vogler B et al. Meningeal blood flow is controlled by H2 S-NO crosstalk activating a HNO-TRPA1-CGRP signalling pathway. Br J Pharmacol. 2016 Feb;173(3):431-45

Rodionova K, Fiedler C, Guenther F et al. Complex reinnervation pattern after unilateral renal denervation in rats. Am J Physiol Regul Integr Comp Physiol 310: R806–R818, 2016

Zhang Y, Yang J, Zhang P et al. Calcitonin gene-related peptide is a key factor in the homing of transplanted human MSCs to sites of spinal cord injury. Sci Rep. 2016 Jun 14;6:27724

Abu Bakar H, Dunn WR, Daly C et al. Sensory innervation of perivascular adipose tissue: a crucial role in artery vasodilatation and leptin release. Cardiovasc Res. 2017 Mar 23

Sastre E, Caracuel L, Prieto I et al. Decompensated liver cirrhosis and neural regulation of mesenteric vascular tone in rats: role of sympathetic, nitrergic and sensory innervations. Sci Rep. 2016 Aug 3;6:31076

Ghadhanfar E, Al-Bader M, Turcani M. Wistar rats resistant to the hypertensive effects of ouabain exhibit enhanced cardiac vagal activity and elevated plasma levels of calcitonin gene-related peptide. PLoS One. 2014 Oct 3;9(10):e108909

Technical File

MSDS