Prolactin (rat) ELISA kit

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Cat No: A05101

Assay Kits - Elisa

€416.00

Price is excluding VAT and does not include packaging neither shipping

Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...

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Product Overview
Data
Scientific Literature
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                        Territorial Availability: Available worldwide directly through Bertin or your local distributor
                    

                                            Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
                    

Synonyms:

Luteotropic hormone

Correlated keywords:

ELISA

enzyme

immunoassays

ELISA

spi-bio

spibio

A05101

Lactation

reproduction

immune

function

osmoregulation

metabolism

Luteotropic

rodent

Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Prolactin (PRL) is a pituitary hormone whose molecular weight is approximately 23000 Daltons. It is a single polypeptide chain composed of about 200 amino acid residues with three disulphide bonds. In mammals prolactin has been claimed to exert a wide range of different physiological effects. These include stimulation of mammary gland development and lactation, hair maturation, synergism with androgen in male sex accessory growth and maintenance and secretion of corpus luteum. PRL is predominantly under inhibitory control by the hypothalamus. Stimulation of prolactin release can be mediated by dopamine and thyrotrophin-releasing hormone(TRH).

Size	96 wells
Shipping	Dry ice
Specificity	Rat Prolactin: 100% Rat GH, Lh or TSH: <1% Murine Prolactin: <10%
Application Media	Plasma, serum and culture supernatant\|No extraction required
Sample volume	50 µL
Tracer	AcetylCholinesterase AChE
Detection Limit	0,2 ng/mL
Standard Curve Range	0,4-50 ng/mL
Custom Code	3822000000
UNSPSC code	41116104

PRODUCT EXPLANATION: ACETYLCHOLINESTERASE MARKER IN ENZYME-IMMUNOASSAYS

Grassi J., Pradelles P. Compounds labelled by the acetylcholinesterase of Electrophorus Electricus. Its preparation process and its use as a tracer or marquer in enzymo-immunological determinations. United States patent, N° 1,047,330. September 10, 1991

Grassi J., Pradelles P. The use of Acetylcholinesterase as a Universal marker in Enzyme-Immunoassays. Proceedings of the Third International Meeting on Cholinesterases, American Chemical Society (1991)

Pradelles P., Grassi J., Maclouf J. Enzyme Immunoassays of Eicosanoids Using Acetylcholinesterase. Methods in Enzymology (1990), vol. 187, 24-34

PRODUCT EXPLANATION : PROLACTIN

Freeman M.E, Kanyicska B., Lerant A. et al. Prolactin: Structure, Function, and Regulation of Secretion. Physiological Reviews, Vol. 80, No. 4, October 2000

PRODUCT EXPLANATION: KIT DEVELOPMENT

Duhau L, Grassi J, Grouselle D; et al. An Enzyme Immunoassay for rat Prolactin: application to the determination of plasma levels. J. Immunoassay, 12(2), 233-250, 1991

Valentin MA, Ma S, Zhao A, Legay F, Avrameas A. Validation of immunoassay for protein biomarkers: Bioanalytical study plan implementation to support pre-clinical and clinical studies. J Pharm Biomed Anal. (2011) 55(5): 869-877

European Medicines Agency. Guideline on bioanalytical method validation, 21 July 2011

PRODUCT CITATIONS

Nikishina Y.O., Sapronova A.Y., Ugrumov M.V. The Effect of Dopamine Secreted by the Brain into the Systemic Circulation on Prolactin Synthesis by the Pituitary gland in Ontogenesis. Acta Naturae, vol. 8 № 3 (30) 2016

Suzuki Y., Nakahara K., Maruyama K. et al. Changes in mRNA expression of arcuate nucleus appetite-regulating peptides during lactation in rats. Journal of Molecular Endocrinology; April 1, 2014; vol.52; 97-109

Capasso R. Effect of Silitidil, a Standardized Extract of Milk Thistle, on the Serum Prolactin Levels in Female Rats. Natural Product Communications Vol. 9 (7) 2014

Kanisra Mary F., Eswaramohan T., Surendran S.N. et al. Mukkuddu Maathrai (Triple Tablet), An Indigenous Medicine Elevates Serum Prolactin Level Of Female Rats (Rattus norvegicus). IOSR Journal Of Pharmacy, Volume 3, Issue (January 2013), PP.38-42

Calejo A. I., Jorgacevski J., Silva V.S. et al. Aluminium-induced changes of fusion pore properties attenuate prolactin secretion in rat pituitary lactotrophs. Neuroscience 201 (2012), 57-66

Pujianto D.A, Curry B.J. and Aitken R.J. Prolactin Exerts a Prosurvival Effect on Human Spermatozoa via Mechanisms that Involve the Stimulation of Akt Phosphorylation and Suppression of Caspase Activation and Capacitation. Endocrinology, March 2010, 151(3):1269–1279

Miyoshi T., Otsuka F., Otani H. et al. Involvement of bone morphogenetic protein-4 in GH regulation by octreotide and bromocriptine in rat pituitary GH3 cells. Journal of Endocrinology (2008) 197, 159-169

Technical File

MSDS