The affinity sorbent A19482 is produced by our labs; it is an immobilized mouse monoclonal antibody anti-CGRP on a matrix resin. The vial contains 1mL of sorbent which can treat up to 20mL sample volume. The use of sorbent will provide you with CGRP-fr...More
The following is the purification protocol to generate CGRP-free biological samples
Put 1 mL of sorbent in a tube.
Add up to 20 mL of plasma or biological sample.
Let it incubate overnight at +4°C with gentle agitation.
Pour the mixture in an empty column (around 10 x 1 cm) with a filter at the end.
Remove the bottom cap and let the plasma or biological sample elute entirely (the gel should become dry but not for a long time).
The eluted plasma or biological sample is now free of CGRP and ready to be used to dilute standards and samples in the assay.