CGRP (human) ELISA kit

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Cat No: A05481

Assay Kits - Elisa

Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...

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Product Overview
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Scientific Literature
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                        Territorial Availability: Available worldwide directly through Bertin or your local distributor
                    

                                            Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
                    

Synonyms:

Calcitonin Gene-Related Peptide (human) ELISA kit

Correlated keywords:

calcitonin

peptide

measures

enzyme

immunoassays

ELISA

neurochemistry

neuroscience

vasodilator

pain

migraine

spi-bio

spibio

A05481

CGRP

calcitonin receptor-like receptor (CLR or CALCRL)

receptor activity modifying protein (RAMP)

589101

Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Calcitonin Gene Related Peptide (CGRP) is a potent vasodilator, and also elicits a number of other biological effects. Average plasma levels of CGRP have been reported to be from 0.8 pmol/L to 71 pmol/l (3 pg/mL to 269 pg/mL) in normal subjects. Increases in circulating CGRP levels have been noticed during hemodialysis, pregnancy, exacerbation of asthma, and in cases of medullary thyroid carcinoma.

Size	96 wells
Shipping	wet ice
Specificity	Human CGRP-I/II: 100% Human CGRP-α/β: 100% Rat CGRP I/II: 120% Rat CGRP α/β: 120% CGRP (8-37): <0.01 % Amylin: <0.01% Calcitonin: <0.01% Substance P: <0.01 %
Application Media	Human blood, plasma, serum, culture supernatant, nervous tissues, cerebrospinal fluid\|Plasma and serum: no extraction required if free plasma or serum used for standard curve.\|Other nervous tissues: extraction with 2N acetic acid.\|Blood and other mixtures
Sample volume	100 µL
Tracer	AcetylCholinesterase AChE
Detection Limit	2 pg/mL
Standard Curve Range	8-1000 pg/mL
Custom Code	3822.00
UNSPSC code	41116104

PRODUCT EXPLANATION: ACETYLCHOLINESTERASE MARKER IN ENZYME-IMMUNOASSAYS

Grassi J., Pradelles P. Compounds labelled by the acetylcholinesterase of Electrophorus Electricus. Its preparation process and its use as a tracer or marquer in enzymo-immunological determinations. United States patent, N° 1,047,330. September 10, 1991

Grassi J., Pradelles P. The use of Acetylcholinesterase as a Universal marker in Enzyme-Immunoassays. Proceedings of the Third International Meeting on Cholinesterases, American Chemical Society (1991)

Pradelles P., Grassi J., Maclouf J. Enzyme Immunoassays of Eicosanoids Using Acetylcholinesterase. Methods in Enzymology (1990), vol. 187, 24-34

PRODUCT EXPLANATION: CGRP

Russell FA, King R, Smillie SJ et al. Calcitonin gene-related peptide: physiology and pathophysiology. Physiol Rev. 2014 Oct;94(4):1099-142

Schou WS, Ashina S, Amin FM et al. Calcitonin gene-related peptide and pain: a systematic review. J Headache Pain. 2017 Dec;18(1):34

PRODUCT EXPLANATION: CGRP KIT DEVELOPMENT

Frobert Y., Nevers M.-C., Amadesi S., Volland H., Brune P., Geppetti P., Grassi J. & Créminon C A sensitive sandwich enzyme immunoassay for calcitonin gene-related peptide (CGRP): characterization and application. Peptide 20 (1999) 275-284

Valentin MA, Ma S, Zhao A, Legay F, Avrameas A. Validation of immunoassay for protein biomarkers: Bioanalytical study plan implementation to support pre-clinical and clinical studies. J Pharm Biomed Anal. (2011) 55(5) : 869-877

European Medicines Agency. Guideline on bioanalytical method validation, 21 July 2011

PRODUCT CITATIONS

Van Gerven L, Alpizar YA, Wouters MM et al. Capsaicin treatment reduces nasal hyperreactivity and transient receptor potential cation channel subfamily V, receptor 1 (TRPV1) overexpression in patients with idiopathic rhinitis. J Allergy Clin Immunol. 2014 May;133(5):1332-9, 1339.e1-3

Matsuo K, Nakagawa H, Adachi Y et al. Photoinduced Upregulation of Calcitonin Gene-Related Peptide in A549 Cells through HNO Release from a Hydrophilic Photocontrollable HNO Donor. Chem Pharm Bull (Tokyo). 2012;60(8):1055-62

Matsuda R, Kezuka T, Nishiyama C et al. Suppression of Murine Experimental Autoimmune Optic Neuritis by Mature Dendritic Cells Transfected with Calcitonin Gene–Related Peptide Gene. Invest Ophthalmol Vis Sci. 2012 Aug 13;53(9):5475-85. doi: 10.1167/iovs.12-9935

Burns LE, Ramsey AA, Emrick JJ et al. Variability in Capsaicin-Stimulated CGRP Release from Human Dental Pulp. J Endod. 2016 Apr;42(4):542-6

Roggenkamp D, Köpnick S, Stäb F et al. Epidermal Nerve Fibers Modulate Keratinocyte Growth via Neuropeptide Signaling in an Innervated Skin Model. J Invest Dermatol. 2013 Jun;133(6):1620-8

Buvanendran A, Kroin JS, Della Valle CJ et al. Cerebrospinal Fluid Neurotransmitter Changes During the Perioperative Period in Patients Undergoing Total Knee Replacement: A Randomized Trial. Anesth Analg. 2012 Feb;114(2):434-41

Song EY, Vandunk C, Kuddo T et al. Measurement of CGRP in dried blood spots using a modified sandwich enzyme immunoassay. J Neurosci Methods. 2006 Jul 15;155(1):92-7. Epub 2006 Feb 8.

Technical File

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