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20-Hydroxyecdysone ELISA kit
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Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...
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Territorial Availability: Available worldwide directly through Bertin or your local distributor
Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
Synonyms:
- 20E
- insect molting hormone
- 20-OH ecdysone
- β-ecdysone
- β-ecdysterone
- crustecdysterone
- ecdysterone
- 20-β-hydroxyecdysterone
Correlated keywords:
- ELISA
- spi-bio
- hormone
- ecdysteroid
- 20-hydroxyecdysone
- 20E
- insect
- moulting
Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Ecdysone is a steroid hormone of the ecdysteroids family which is found in animals (vertebrates and invertebrates) and in plants. There exist different polyhydroxylated forms of ecdysone, the major one being the 20-Hydroxyecdysone. 20-Hydroxyecdysone (20E) is known as the insect moulting hormone. Indeed, in interaction with the juvenile hormone (JH), 20E plays a key role in growth, development, metamorphosis and reproduction of the Arthropods (i.e. invertebrates such as insects, crustaceans, arachnids, etc.).
Little is known about the function of ecdysteroids in the vertebrates. There are several reports which indicate an effect of the phycoecdysteroid 20E on muscle growth and fat loss in mammals. Some dietary supplements are marketed to enhance physical performances of athletes, and particularly of bodybuilders. Besides, researchers are currently studying the effect of 20E in the treatment of obesity and diabetes. And there are on-going investigations on the possible interest of 20E in the prevention and the treatment of diseases such as osteoporosis and osteoarthritis.
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Ecdysone is a steroid hormone of the ecdysteroids family which is found in animals (vertebrates and invertebrates) and in plants. There exist different polyhydroxylated forms of ecdysone, the major one being the 20-Hydroxyecdysone. 20-Hydroxyecdysone (20E) is known as the insect moulting hormone. Indeed, in interaction with the juvenile hormone (JH), 20E plays a key role in growth, development, metamorphosis and reproduction of the Arthropods (i.e. invertebrates such as insects, crustaceans, arachnids, etc.).
Little is known about the function of ecdysteroids in the vertebrates. There are several reports which indicate an effect of the phycoecdysteroid 20E on muscle growth and fat loss in mammals. Some dietary supplements are marketed to enhance physical performances of athletes, and particularly of bodybuilders. Besides, researchers are currently studying the effect of 20E in the treatment of obesity and diabetes. And there are on-going investigations on the possible interest of 20E in the prevention and the treatment of diseases such as osteoporosis and osteoarthritis.
| Size | 96 wells |
| Shipping | Dry Ice |
| Specificity | |
| Application Media | extraction with ethanol or methanol for biological samples like haemolymph, insect larvae... |
| Sample volume | 50 µL |
| Tracer | AcetylCholinesterase AChE |
| Standard Curve Range | 39 - 5000 pg/mL |
| Custom Code | 3822.00 |
| UNSPSC code | 41116104 |
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PRODUCT EXPLANATION: ACETYLCHOLINESTERASE MARKER IN ENZYME-IMMUNOASSAYS
Grassi J., Pradelles P. Compounds labelled by the acetylcholinesterase of Electrophorus Electricus. Its preparation process and its use as a tracer or marquer in enzymo-immunological determinations. United States patent, N° 1,047,330. September 10, 1991
Grassi J., Pradelles P. The use of Acetylcholinesterase as a Universal marker in Enzyme-Immunoassays. Proceedings of the Third International Meeting on Cholinesterases, American Chemical Society (1991)
Pradelles P., Grassi J., Maclouf J. Enzyme Immunoassays of Eicosanoids Using Acetylcholinesterase. Methods in Enzymology (1990), vol. 187, 24-34
PRODUCT EXPLANATION: 20E KIT DEVELOPMENT AND SAMPLE PREPARATION
Porcheron P., Morinière M., Grassi J. and P. Pradelles. Development of an enzyme immunoassay for ecdysteroids using acetylcholinesterase as label. Insect Biochemistry, vol.19, No.2, p.117-122, 1989
Pascual N., Bellés X. et al. Quantification of ecdysteroids by immunoassay : comparison of enzyme immunoassay and radioimmunoassay. Zeitschrift für Naturforschung, 50c, 862-867, 1995
Claret J., Porcheron P. and Dray F. Critical period and ecdydone titers in the pupae of Pieris brassicae. Separatum Experientia 33, 1389, 1977
Porcheron P., Foucrier J., Gros Cl., Pradelles P.,et al. Radioimmunoassay of arthropod moulting hormone: β−ecdysone antibodies production and 125I-Iodinated tracer preparation. FEBS Letters, vol. 61, No.2, p.159, 1976
Porcheron P., Cassier P. and Dray F. Applications de techniques immunochimiques à l’étude de l’ecdysone. Bull. Soc. Zool. France, Suppl.1, p71-75, 1977
Valentin MA, Ma S, Zhao A, Legay F, Avrameas A. Validation of immunoassay for protein biomarkers: Bioanalytical study plan implementation to support pre-clinical and clinical studies. J Pharm Biomed Anal. (2011) 55(5) : 869-877
European Medicines Agency. Guideline on bioanalytical method validation, 21 July 2011
PRODUCT CITATIONS
Planelló R, Herrero Ó, Gómez-Sande P et al. Ecdysone-Related Biomarkers of Toxicity in the Model Organism Chironomus riparius: Stage and Sex-Dependent Variations in Gene Expression Profiles. PLoS One. 2015 Oct 8;10(10):e0140239
Christesen D, Yang YT,Somers J et al. Transcriptome Analysis of Drosophila melanogaster Third Instar Larval Ring Glands Points to Novel Functions and Uncovers a Cytochrome p450 Required for Development. G3: Genes, Genomes, Genetics (Bethesda), Vol. 7, No. 2, 2017, p. 467-479
Lafontaine A, Hanikenne M, Boulangé-Lecomte C et al. Vitellogenin and vitellogenin receptor gene expression and 20-hydroxyecdysone concentration in Macrobrachium rosenbergii exposed to chlordecone. Environ Sci Pollut Res Int. 2016 Oct;23(20):20661-20671
Li K, Tian L, Guo Z et al. 20-Hydroxyecdysone (20E) Primary Response Gene E75 Isoforms Mediate Steroidogenesis Autoregulation and Regulate Developmental Timing in Bombyx. J Biol Chem. 2016 Aug 26;291(35):18163-75
Gabrieli P, Kakani EG, Mitchell SN et al. Sexual transfer of the steroid hormone 20E induces the postmating switch in Anopheles gambiae. Proc Natl Acad Sci U S A. 2014 Nov 18;111(46):16353-8
Koyama T, Rodrigues MA, Athanasiadis A et al. Nutritional control of body size through FoxO-Ultraspiracle mediated ecdysone biosynthesis. Elife. 2014 Nov 25;3
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