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- 20-Hydroxyecdysone ELISA kit
20-Hydroxyecdysone ELISA kit
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Cat No: A05120
Assay Kits
- Elisa
€468.00
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...
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Territorial Availability: Available worldwide directly through Bertin or your local distributor
Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
Synonyms:
- 20E
- insect molting hormone
- 20-OH ecdysone
- β-ecdysone
- β-ecdysterone
- crustecdysterone
- ecdysterone
- 20-β-hydroxyecdysterone
Correlated keywords:
- ELISA
- spi-bio
- hormone
- ecdysteroid
- 20-hydroxyecdysone
- 20E
- insect
- moulting
Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Ecdysone is a steroid hormone of the ecdysteroids family which is found in animals (vertebrates and invertebrates) and in plants. There exist different polyhydroxylated forms of ecdysone, the major one being the 20-Hydroxyecdysone. 20-Hydroxyecdysone (20E) is known as the insect moulting hormone. Indeed, in interaction with the juvenile hormone (JH), 20E plays a key role in growth, development, metamorphosis and reproduction of the Arthropods (i.e. invertebrates such as insects, crustaceans, arachnids, etc.).
Little is known about the function of ecdysteroids in the vertebrates. There are several reports which indicate an effect of the phycoecdysteroid 20E on muscle growth and fat loss in mammals. Some dietary supplements are marketed to enhance physical performances of athletes, and particularly of bodybuilders. Besides, researchers are currently studying the effect of 20E in the treatment of obesity and diabetes. And there are on-going investigations on the possible interest of 20E in the prevention and the treatment of diseases such as osteoporosis and osteoarthritis.
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Ecdysone is a steroid hormone of the ecdysteroids family which is found in animals (vertebrates and invertebrates) and in plants. There exist different polyhydroxylated forms of ecdysone, the major one being the 20-Hydroxyecdysone. 20-Hydroxyecdysone (20E) is known as the insect moulting hormone. Indeed, in interaction with the juvenile hormone (JH), 20E plays a key role in growth, development, metamorphosis and reproduction of the Arthropods (i.e. invertebrates such as insects, crustaceans, arachnids, etc.).
Little is known about the function of ecdysteroids in the vertebrates. There are several reports which indicate an effect of the phycoecdysteroid 20E on muscle growth and fat loss in mammals. Some dietary supplements are marketed to enhance physical performances of athletes, and particularly of bodybuilders. Besides, researchers are currently studying the effect of 20E in the treatment of obesity and diabetes. And there are on-going investigations on the possible interest of 20E in the prevention and the treatment of diseases such as osteoporosis and osteoarthritis.
| Size | 96 wells |
| Shipping | Dry Ice |
| Stability | Store at -20degrees; shelf life 1 year maximum after production |
| Specificity | |
| Application Media | extraction with ethanol or methanol for biological samples like haemolymph, insect larvae... |
| Sample volume | 50 µL |
| Tracer | AcetylCholinesterase AChE |
| Standard Curve Range | 39 - 5000 pg/mL |
| Custom Code | 3822.00 |
| UNSPSC code | 41116104 |
PRODUCT EXPLANATION: ACETYLCHOLINESTERASE MARKER IN ENZYME-IMMUNOASSAYS
Grassi J., Pradelles P. Compounds labelled by the acetylcholinesterase of Electrophorus Electricus. Its preparation process and its use as a tracer or marquer in enzymo-immunological
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