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SREBP-2 Transcription Factor Assay Kit

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    SREBP-<wbr/>2 Transcription Factor Assay Kit
  • SREBP-<wbr/>2 Transcription Factor Assay Kit
Cat No: 10007819
Assay Kits - Colorimetric Assays

Lipid homeostasis in vertebrate cells is regulated by a family of transcription factors called sterol regulatory element-binding proteins (SREBP’s). SREBP’s directly activate the expression of over 30 genes involved in the synthesis and uptake of chol...

: 96 wells

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Territorial Availability: Available through Bertin Technologies only in France
Correlated keywords:
  • tf sterol regulatory element binding proteins cholesterol fatty acids triglycerides phospholipids atherosclerosis assays
Product Overview:
Lipid homeostasis in vertebrate cells is regulated by a family of transcription factors called sterol regulatory element-binding proteins (SREBP’s). SREBP’s directly activate the expression of over 30 genes involved in the synthesis and uptake of cholesterol, fatty acids, triglycerides, and phospholipids.{14287} Three different SREBP isoforms designated SREBP-1a, SREBP-1c, and SREBP-2 are encoded by two different genes and belong to the basic helix-loop-helix-leucine zipper (bHLH-ZIP) family of transcription factors.{14286} SREBP-2 performs a critical role in the transcriptional regulation of genes involved in cholesterol synthesis and uptake including HMG-CoA synthase, HMG-CoA reductase, and the LDL receptor. Cayman’s SREBP-2 Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A 96 well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the SREBP response element is immobilized to the wells of a 96 well plate. SREBP contained in a nuclear extract, binds specifically to the SREBP response element. SREBP is detected by addition of specific primary antibody directed against SREBP. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm.
Size 96 wells
Shipping dry ice
Custom Code 3822.19
UNSPSC code 41116104

Brown, M.S., and Goldstein, J.L. The SREBP pathway: Regulation of cholesterol metabolism by proteolysis of a membrane-bound transcription factor. Cell 89 331-340 (1997).

Osborne, T.F. Sterol regulatory element-binding proteins (SREBP’s): Key r

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Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

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