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MitoCheck® Complex I Activity Assay Kit

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    MitoCheck<sup>®</sup> Complex I Activity Assay Kit
  • MitoCheck<sup>®</sup> Complex I Activity Assay Kit
Cat No: 700930
Assay Kits - Colorimetric Assays

Complex I (NADH oxidase/Co-enzyme Q reductase) is one of the major sites of electron entry into the mitochondrial electron transport chain (ETC). Complex I catalyzes the 2 electron oxidation of NADH followed by the reduction of ubiquinone (Q) to form ...

: 96 wells

This product can only be bought through Cayman Chemical. Please contact us.

Territorial Availability: Available through Bertin Technologies only in France
Correlated keywords:
  • kits assays measures measurements 1 one nicotinamides adenines dinucleotides reduced NADH oxidases Co-enzymes Q oxidases/Co-enzymes CoQ10 reductases e- electrons mitochondrial mitochondrias transports chains ETCs catalyzes two 2 II oxidations reduces reductions ubiquinones ubiquinols QH2 terminal acceptors oxygens O2 translocations translocalizations 4 four protons H+ matrix matrices intermembrane spaces chemiosmotic gradients oxidative phosphorylations phosphorylates inhibits inhibitors inhibitions dysfunctions produces productions reactive species ROSs neurological brains disorders Parkinson's Parkinsons diseases Down's Downs Leigh's Leighs syndromes indicates indicators defects toxicity toxicities effects rotenone piericidin isolates isolations
Product Overview:
Complex I (NADH oxidase/Co-enzyme Q reductase) is one of the major sites of electron entry into the mitochondrial electron transport chain (ETC). Complex I catalyzes the 2 electron oxidation of NADH followed by the reduction of ubiquinone (Q) to form ubiquinol (QH2), and ultimately the reduction of the terminal electron acceptor, O2. During the passage of electrons from NADH to Q, the translocation of four protons (H+) from the mitochondrial matrix to the intermembrane space occurs, contributing to the chemiosmotic proton gradient, which is required for oxidative phosphorylation. Cayman's MitoCheck® Complex I Activity Assay allows for the activity of complex I to be determined without the need to isolate mitochondria or pre-incubate with antibodies. The rate of NADH oxidation is measured by a decrease in absorbance at 340 nm and is proportional to the activity of complex I. For the use of this kit with other types of tissue mitochondria, please see Cayman's MitoCheck® Mitochondrial (Tissue) Isolation Kit (Item No. 701010).
Size 96 wells
Shipping dry ice
Custom Code 3822.19
UNSPSC code 41116104

Papa, S., and De Rasmo, D. Complex I deficiencies in neurological disorders. Trends Mol Med 19(1) 61-69 (2013).

Janetzky, B., Hauck, S., Youdim, M.B.H., et al. Unaltered aconitase activity, but decreased complex I activity in substantia nigra pars compact

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Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

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