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MitoCheck® Complex II/III Activity Assay Kit

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    MitoCheck<sup>®</sup> Complex II/III Activity Assay Kit
  • MitoCheck<sup>®</sup> Complex II/III Activity Assay Kit
Cat No: 700950
Assay Kits - Colorimetric Assays

Complex III (CoQ cytochrome c oxidoreductase) is an essential protein for mitochondrial oxidative phosphorylation. Complex III functions as both a gatekeeper for mitochondrial respiration and as a major source of reactive oxygen species III. It accept...

: 96 wells

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Territorial Availability: Available through Bertin Technologies only in France
Correlated keywords:
  • kits assays measures measurements measuring 3 three CoQ coenzymes Q10 cytochromes c oxidoreductases proteins essential oxidatives phosphorylates phosphorylations gatekeeper respirations reactive oxygens species ROSs acceptor electrons e- complexes 1 2 I II one two QH2 ubiquinol ubiquinone carrier bound binds redox reactions reductions oxidations Q-cycles Q cycles Reiske irons sulfurs iron-sulfur Fe-S 2Fe-2S 4Fe-4S 3Fe-4S clusters C1 translocates translocations 4H+ O2- coupled mechanisms inhibits inhibitors inhibitions excess isolates isolations rotenone potassium cyanides KCN
Product Overview:
Complex III (CoQ cytochrome c oxidoreductase) is an essential protein for mitochondrial oxidative phosphorylation. Complex III functions as both a gatekeeper for mitochondrial respiration and as a major source of reactive oxygen species III. It accepts electrons from complexes I and II in the form of QH2, the reduced form of the electron carrier ubiquinone. Once bound to complex III, QH2 undergoes a series of redox reactions, known as the Q-cycle. During the Q-cycle, electrons are passed from QH2 onto cytochrome c via the Rieske iron-sulfur protein and cytochrome c1 resulting in the translocation of 4H+ and the generation of 02•-. Cayman's MitoCheck® Complex II/III Activity Assay measures the reduction of excess cytochrome c (550 nm absorbance) as catalyzed by complex III. This assay is coupled to succinate co-enzyme Q oxidoreductase (complex II) for the generation of QH2. Due to the dependence on complex II activity, a counter-screen for complex II activity (Item No. 700940) should be performed in order to truly measure complex III activity. For the use of this kit with other types of tissue mitochondria, please see Cayman's MitoCheck® Mitochondrial (Tissue) Isolation Kit (Item No. 701010).
Size 96 wells
Shipping dry ice
Custom Code 3822.19
UNSPSC code 41116104

St-Pierre, J., Buckingham, J.A., Roebuck, S.J., et al. Topology of superoxide production from different sites in the mitochondrial electron transport chain. J Biol Chem 277(47) 44784-44790 (2002).

Lambert, A.J., and Brand, M.D. Inhibitors of the qui

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Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

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