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Obestatin (human) ELISA kit

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    Obestatin (human) ELISA kit
  • Obestatin (human) ELISA kit
Cat No: A05036
Assay Kits - Elisa
Bertin Bioreagent
€432.00
Price is excluding VAT and does not include packaging neither shipping

Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...

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: 96 wells

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Territorial Availability: Available worldwide directly through Bertin or your local distributor
Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
Correlated keywords:
  • ELISA
  • Obestatin
  • preproghrelin peptide
  • Acyl ghrelin
  • desacyl ghrelin
  • obesity
  • intestinal peptide
  • diabete
Show more...
Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Obestatin is an amidated peptide of 23 amino-acids with an alpha-helical secondary conformation. It was first isolated from rat stomach in 2005. Obestatin is a preproghrelin-derived peptide produced by many tissues or organs, including the stomach, pancreas, adipose tissue, skeletal muscle, and heart. Obestatin was originally identified as an anorexigenic peptide that reduces food intake and body weight by opposing the actions of ghrelin. It has also been considered to be an antidiabetic peptide that positively influences glucose and lipid metabolism. It may also play a role in the regulation of blood pressure as its plasma concentration increases in hypertensive patients. Obestatin may also have a role in the regulation of anxiety and memory improvement.
Size 96 wells
Shipping dry ice
Specificity
Application Media Plasma (diluted at 1/8)| buffer
Sample volume 100 µL
Tracer AcetylCholinesterase AChE
Detection Limit 62,5 pg/mL
Standard Curve Range 31,3 pg/mL - 4 000,0 pg/mL
Custom Code 3822000000
UNSPSC code 41116104

PRODUCT EXPLANATION: ACETYLCHOLINESTERASE MARKER IN ENZYME-IMMUNOASSAYS

Grassi J., Pradelles P. Compounds labelled by the acetylcholinesterase of Electrophorus Electricus. Its preparation process and its use as a tracer or marquer in enzymo-immunological determinations. United States patent, N° 1,047,330. September 10, 1991

Grassi J., Pradelles P. The use of Acetylcholinesterase as a Universal marker in Enzyme-Immunoassays. Proceedings of the Third International Meeting on Cholinesterases, American Chemical Society (1991)

Pradelles P., Grassi J., Maclouf J. Enzyme Immunoassays of Eicosanoids Using Acetylcholinesterase. Methods in Enzymology (1990), vol. 187, 24-34

PRODUCT EXPLANATION: OBESTATIN

Alen B, Nieto L, Gurriaran-Rodriguez U et al. The NMR Structure of Human Obestatin in Membrane-Like Environments: Insights into the Structure-Bioactivity Relationship of Obestatin. PLOS ONE

www.plosone.org, vol. 7, Issue 10, e45434 2012

Zhang J.V, Ren P.G, Avsian-Kretchmer O et al. Obestatin, a peptide encoded by the ghrelin gene, opposes ghrelin’s effects on food intake. Science 310 996-999, 2005

Gronberg M, Tsolakis AV, Magnusson L et al. Distribution of obestatin and ghrelin in human tissues: immunoreactive cells in the gastrointestinal tract, pancreas, and mammary glands. J Histochem Cytochem ;56 :793-801, 2008

Granata R, Baragli A, Settanni F et al. Unraveling the role of the ghrelin gene peptides in the endocrine pancreas. J Mol Endocrinol ;45 :107-118, 2010

Ren AJ, He Q, SHI JS et al. Association of obestatin with blood pressure in the third trimesters of pregnancy. Peptides ;30 :1742-1745 ;2009

Li ZF, Guo ZF, Cao J et al. Plasma ghrelin and obestatin levels are increased in spontaneously hypertensive rats. Peptides ;31 :297-300 ;2010

Carlini VP, Schioth HB and Debarioglio SR. Obestatin improves memory performance and causes anxiolytic effects in rats. Biochem Biophys Res Commun ;352 :907-12, 2007

PRODUCT EXPLANATION: KIT DEVELOPMENT

Valentin MA, Ma S, Zhao A et al. Validation of immunoassay for protein biomarkers: Bioanalytical study plan implementation to support pre-clinical and clinical studies. J Pharm Biomed Anal. (2011) 55(5): 869-877

European Medicines Agency. Guideline on bioanalytical method validation, 21 July 2011

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