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ANP (rat) ELISA kit
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Cat No: A05103
Assay Kits
- Elisa
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...
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Territorial Availability: Available worldwide directly through Bertin or your local distributor
Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
Synonyms:
- Atrial natriuretic peptide (ANP)
- Atrial natriuretic factor (ANF)
- Atrial natriuretic hormone (ANH)
- Cardionatrine
- Cardiodilatin (CDD)
Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Atriopeptin is a 28 amino acid peptide synthesised primarily in cardiac atria. This peptide hormone acts in an opposing manner to angiotensin in regulating renal, hemodynamic and endocrine function. Elevated plasma levels may be produced in experimental models by volume expansion, high salt diets, and vasoconstrictors. Increased plasma concentrations have also been reported in various pathological conditions such as renal disease, congestive heart failure, and paroxysmal atrial tachycardia.
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Atriopeptin is a 28 amino acid peptide synthesised primarily in cardiac atria. This peptide hormone acts in an opposing manner to angiotensin in regulating renal, hemodynamic and endocrine function. Elevated plasma levels may be produced in experimental models by volume expansion, high salt diets, and vasoconstrictors. Increased plasma concentrations have also been reported in various pathological conditions such as renal disease, congestive heart failure, and paroxysmal atrial tachycardia.
| Size | 96 wells |
| Shipping | dry ice |
| Specificity | Show more... |
| Application Media | Plasma and cardia tissue|Solid-phase extraction for plasma sample.|Cardia tissue: extraction with CH3COOH |
| Sample volume | 50 µL |
| Tracer | AcetylCholinesterase AChE |
| Detection Limit | 16 pg/mL |
| Standard Curve Range | 16-2000 pg/mL |
| Custom Code | 3822000000 |
| UNSPSC code | 41116104 |
PRODUCT EXPLANATION: ACETYLCHOLINESTERASE MARKER IN ENZYME-IMMUNOASSAYS
Grassi J., Pradelles P. Compounds labelled by the acetylcholinesterase of Electrophorus Electricus. Its preparation process and its use as a tracer or marquer in enzymo-immunological determinations. United States patent, N° 1,047,330. September 10, 1991
Grassi J., Pradelles P. The use of Acetylcholinesterase as a Universal marker in Enzyme-Immunoassays. Proceedings of the Third International Meeting on Cholinesterases, American Chemical Society (1991)
Pradelles P., Grassi J., Maclouf J. Enzyme Immunoassays of Eicosanoids Using Acetylcholinesterase. Methods in Enzymology (1990), vol. 187, 24-34
PRODUCT EXPLANATION: ATRIOPEPTIN
Saito Y. Roles of atrial natriuretic peptide and its therapeutic use. Journal of Cardiology (2010) 56, 262—270
PRODUCT EXPLANATION: KIT DEVELOPMENT
McLaughlin LL, Wei YF, Stockmann PT et al. Development, validation and application of an enzyme immunoassay (EIA) of atriopeptin. Biochem Biophys Res Commun. 1987 Apr 14;144(1):469-76
Valentin MA, Ma S, Zhao A, Legay F, Avrameas A. Validation of immunoassay for protein biomarkers: Bioanalytical study plan implementation to support pre-clinical and clinical studies. J Pharm Biomed Anal. (2011) 55(5) : 869-877
European Medicines Agency. Guideline on bioanalytical method validation, 21 July 2011
PRODUCT CITATIONS
Casartelli A, Dacome L, Tessari M et al. Cocaine-associated increase of atrial natriuretic peptides: an early predictor of cardiac complications in cocaine users? Heart Asia. 2014 Jul 8;6(1):100-7
Lee CH, Sun SH, Lin SH, Chen CC. Role of the acid-sensing ion channel 3 in blood volume control. Circ J. 2011;75(4):874-83. Epub 2011 Feb 4
Chan JC, Knudson O, Wu F et al. Hypertension in mice lacking the proatrial natriuretic peptide convertase corin. Proc Natl Acad Sci U S A. 2005 Jan 18;102(3):785-90. Epub 2005 Jan 6
Bełtowski J, Marciniak A, Wójcicka G. Leptin decreases renal medullary Na(+), K(+)-ATPase activity through phosphatidylinositol 3-kinase dependent mechanism. J Physiol Pharmacol. 2004 Jun;55(2):391-407
Beltowski J1, Wójcicka G, Borkowska E. Human leptin stimulates systemic nitric oxide production in the rat. Obes Res. 2002 Sep;10(9):939-46
Kumar M, Behera AK, Lockey RF et al. Atrial natriuretic peptide gene transfer by means of intranasal administration attenuates airway reactivity in a mouse model of allergic sensitization. J Allergy Clin Immunol. 2002 Dec;110(6):879-82
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