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Angiotensin II SPIE-IA kit
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Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...
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Territorial Availability: Available worldwide directly through Bertin or your local distributor
Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
The renin-angiotensin system is essential for the control of blood pressure. Among the different peptides resulting from the proteolytic processing of angiotensinogen, the octapeptide Angiotensin II (AII) is the major hormone involved in the pathophysiology of hypertensive diseases as it mediates vasoconstrictor action. It is the target of choice allowing proper estimation of the renin-angiotensin system.
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
The renin-angiotensin system is essential for the control of blood pressure. Among the different peptides resulting from the proteolytic processing of angiotensinogen, the octapeptide Angiotensin II (AII) is the major hormone involved in the pathophysiology of hypertensive diseases as it mediates vasoconstrictor action. It is the target of choice allowing proper estimation of the renin-angiotensin system.
| Size | 96 wells |
| Shipping | Dry ice |
| Specificity | |
| Application Media | Plasma Sample, culture supernatant|Blood collection with inhibitor cocktail; solid-phase extraction for plasma|Culture supernatants: no extraction required |
| Sample volume | 100 µL |
| Tracer | AcetylCholinesterase AChE |
| Detection Limit | 1 pg/mL |
| Standard Curve Range | 1-125 pg/mL |
| Custom Code | 3822.00 |
| UNSPSC code | 41116104 |
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PRODUCT EXPLANATION: ACETYLCHOLINESTERASE MARKER IN ENZYME-IMMUNOASSAYS
Grassi J., Pradelles P. Compounds labelled by the acetylcholinesterase of Electrophorus Electricus. Its preparation process and its use as a tracer or marquer in enzymo-immunological determinations. United States patent, N° 1,047,330. September 10, 1991
Grassi J., Pradelles P. The use of Acetylcholinesterase as a Universal marker in Enzyme-Immunoassays. Proceedings of the Third International Meeting on Cholinesterases, American Chemical Society (1991)
Pradelles P., Grassi J., Maclouf J. Enzyme Immunoassays of Eicosanoids Using Acetylcholinesterase. Methods in Enzymology (1990), vol. 187, 24-34
PRODUCT EXPLANATION: ANGIOTENSIN II
Paulis L, Unger T. Novel therapeutic targets for hypertension Nat Rev Cardiol. 2010 Aug;7(8):431-41
PRODUCT EXPLANATION: KIT DEVELOPMENT
Volland H, Pradelles P, Ronco P, Azizi M, Simon D, Créminon C, Grassi J. A solid-phase immobilized epitope immunoassay (SPIE-IA) permitting very sensitive and specific measurement of angiotensin II in plasma. J Immunol Methods. 1999 Aug 31;228(1-2):37-47
Pradelles Ph. Immunometric determination of an antigen or hapten. United states patent, N°5,476,770. December 19; 1995
Kohara K, Tabuchi Y, Senanayake P, Brosnihan KB, Ferrario CM. Reassessment of plasma angiotensins measurement: effects of protease inhibitors and sample handling procedures. Peptides. 1991 Sep-Oct;12(5):1135-41
Grassi J, Créminon C, Frobert Y, Etienne E, Ezan E, Volland H, Pradelles P. Two different approaches for developing immunometric assays of haptens. Clin Chem. 1996 Sep;42(9):1532-6
Valentin MA, Ma S, Zhao A, Legay F, Avrameas A. Validation of immunoassay for protein biomarkers: Bioanalytical study plan implementation to support pre-clinical and clinical studies. J Pharm Biomed Anal. (2011) 55(5): 869-877
European Medicines Agency. Guideline on bioanalytical method validation, 21 July 2011
PRODUCT CITATIONS
Efrati S, Berman S, Hamad RA, Siman-Tov Y, Ilgiyaev E, Maslyakov I, Weissgarten J. Effect of captopril treatment on recuperation from ischemia/reperfusion-induced acute renal injury. Nephrol Dial Transplant. 2012 Jan;27(1):136-45
Amit Dagan, Jyothsna Gattineni, Sabeen Habib and Michel Baum. Effect of Prenatal Dexamethasone on Postnatal Serum and Urinary Angiotensin II Levels. Am J Hypertens 2010; 23:420-424
Ying-Hong Feng, Lingyin Zhou, Rongde Qiu and Robin Zeng. Single Mutations at Asn295 and Leu305 in the Cytoplasmic Half of Transmembrane α-Helix Domain 7 of the AT1 Receptor Induce Promiscuous Agonist Specificity for Angiotensin II Fragments: A Pseudo-Constitutive Activity. Molecular Pharmacology August 2005, 68 (2) 347-355
Stevens VA, Saad S, Poronnik P, Fenton-Lee CA, Polhill TS, Pollock CA. The role of SGK-1 in angiotensin II-mediated sodium reabsorption in human proximal tubular cells. Nephrol Dial Transplant. 2008 Jun;23(6):1834-43
Yanes LL, Romero DG, Iles JW, Iliescu R, Gomez-Sanchez C, Reckelhoff JF. Sexual dimorphism in the renin-angiotensin system in aging spontaneously hypertensive rats. Am J Physiol Regul Integr Comp Physiol. 2006 Aug;291(2):R383-90
Yvan-Charvet L, Massiéra F, Lamandé N, Ailhaud G, Teboul M, Moustaid-Moussa N, Gasc JM, Quignard-Boulangé A. Deficiency of angiotensin type 2 receptor rescues obesity but not hypertension induced by overexpression of angiotensinogen in adipose tissue. Endocrinology. 2009 Mar;150(3):1421-8
Van Ginkel a,b, De Haan, et al. Exercise intensity modulates capillary perfusion in correspondence with ACE I/D modulated serum angiotensin II levels. Applied & Translational Genomics 4 (2015) 33–37
Kawaguchi, Takagi et al. Angiotensin II in the Lesional Skin of Systemic Sclerosis Patients Contributes to Tissue Fibrosis Via Angiotensin II Type 1 Receptors. ARTHRITIS & RHEUMATISM, Vol. 50, No. 1, January 2004, pp 216–226
Miceli, Burt et al. Stretch reduces nephrin expression via an angiotensin II-AT1-dependent mechanism in human podocytes: effect of rosiglitazone. Am J Physiol Renal Physiol 298: F381–F390, 2010
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