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Anthrax Edema Factor Assay kit

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    Anthrax Edema Factor Assay kit
  • Anthrax Edema Factor Assay kit
Cat No: A05230
Assays - Ache Kits
Price is excluding VAT and does not include packaging neither shipping

Anthrax is an infectious disease due to Bacillus anthracis, a gram-positive, spore-forming bacterium. Among bioterrorist agents, anthrax is considered as one of the most potent and dangerous bio-agent and has been classifi ed in the A list of the Cente...

: 96 wells
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Territorial Availability: Available worldwide directly through Bertin Technologies or your local distributor
Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
Product Overview:
Anthrax is an infectious disease due to Bacillus anthracis, a gram-positive, spore-forming bacterium. Among bioterrorist agents, anthrax is considered as one of the most potent and dangerous bio-agent and has been classifi ed in the A list of the Centers of Disease Control. The pathogenic power of this bacteria is due to an exotoxin, which is secreted into the host’s system when three nontoxic proteins link together to form a toxin complex. The three parts of the pathogen complex are protective antigen (PA), lethal factor (LF) and edema factor (EF).At molecular level, the PA binds to cell receptors where it is cleaved into PA20 and PA63. PA63 can competitively bind EF or LF or both. The PA-LF complex forms the lethal toxin (LTx) and the PA-EF complex forms the edema toxin (ETx). Once ETx is endocytosed, EF is released in cytosole. EF is a calmoduline and Ca2+ dependent adenylyl cyclase which catalyses the conversion of cytosolic adenosine triphosphate into cyclic adenosine monophosphate (cAMP) leading to edema.
Size: 96 wells
Shipping: dry ice
Stability: Store at -20degrees; shelf life 1 year maximum after production
Application Media: Human an mouse serum|No extraction required
Sample volume: 5 µL
Tracer: AcetylCholinesterase AChE
Detection Limit: 0.647 pg/mL
Standard Curve Range: 1 000 pg/mL - 7.81 pg/mL
Custom Code: 3822000000
UNSPSC code: 41116104

Grassi J, Pradelles P. Compounds labelled by the acetylcholinesterase of Electrophorus Electricus. Its preparation process and its use as a tracer or marquer in enzymo-immunological determinations. United States patent, N° 1,047,330. September 10, 1991

Grassi J, Pradelles P. The use of Acetylcholinesterase as a Universal marker in Enzyme-Immunoassays. Proceedings of the Third International Meeting on Cholinesterases, American Chemical Society (1991)

Pradelles P, Grassi J, Maclouf J. Enzyme Immunoassays of Eicosanoids Using Acetylcholinesterase. Methods in Enzymology (1990), vol. 187, 24-34

Fouet A, Mock M. Regulatory networks for virulence and persistence of Bacillus anthracis. Curr. Opin. Microbiol. (2006), 9, 160-166

Bellan S.E., Turnbull P.C.B., Beyer W., Wayne M. Getz W.M., Effects of Experimental Exclusion of Scavengers from Carcasses of Anthrax-Infected Herbivores on Bacillus anthracis Sporulation, Survival, and Distribution. Appl Environ Microbiol. 2013 Jun; 79(12): 3756–3761

Inglesby TV, Henderson DA, Bartlett JG, Ascher MS, Eitzen E, Friedlander AM, Hauer J, McDade J, Osterholm MT, O’Toole T, Parker G, Perl TM, Russell PK, Tonat K. Anthrax as a biological weapon: medical and public health management. J Am. Med. Assoc. (1999), 281,1735-45

Centers of diseases Control and Prevention and Department of Health and Human Services. Bioterrorism Agents/Diseases. (2008)

Collier RJ. Mechanism of membrane translocation by anthrax toxin: insertion and pore formation by protective antigen. J. Appl. Microbiol. (1999), 87, 283

Duriez E, Goossens P L, Becher F, Ezan E. Femtomolar Detection of the Anthrax Edema Factor in Human and Animal Plasma. Anal. Chem. (2009), 81, 5935–5941

Anthrax in Humans and Animals, 4th edition. Geneva: World Health Organization; 2008

Pradelles P, Grassi J, Chabardes D, Guiso N. Enzyme Immunoassays of Adenosine Cyclic 3’, 5’-Monophosphate and Guanosine Cyclic 3’, 5’-Monophosphate Using Acetylcholinesterase. Analytical chemistry (1989), 61, 447-53

SPI-Bio is a brand name of Bertin Bioreagent.
Bertin Bioreagent includes over 100 employees, chemists, biochemists, immunoanalysts, pharmacists and technicians.
Always at the forefront of innovation and after launching on the market products incorporating in their time innovative technologies (Acetylcholinesterase tracer for EIA), this brand offers a wide and unique range of assays for biomarker Immunoassays (SPI-Bio TM) thanks to a solid knowledge acquired during the last 25 years in developping over 100 biomarkers assays.

To better serve you, we aim to design our products and reagents to address your needs on the expected performance in terms of a possible range of use, but also on operational conditions of use & regulations.
Thanks to our production processes and quality controls, we provide you with products with compliance to specifications.
Our technical support & science teams are available to help you assess their relevance depending on your needs, to specify the conditions of application and their fields of use. Finally, our researchers are ready to provide you with the necessary support by going, if needed, in your laboratories to validate your operating conditions and to solve possible problems.

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