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HDAC Fluorometric Activity Assay Kit

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    HDAC Fluorometric Activity Assay Kit
  • HDAC Fluorometric Activity Assay Kit
Cat No: 10011563
Assays - Enzymatic Kits
Cayman
€344.00
Price is excluding VAT and does not include packaging neither shipping

HDACs catalyze the hydrolytic removal of acetyl groups from histone lysine residues resulting in chromatin condensation and transcriptional repression of chromosomal DNA. Thus, HDAC inhibition allows the conformation of DNA to be relaxed and transcript...

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: 96 wells

This product can only be bought through Cayman Chemical. Please contact us.

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Territorial Availability: Available through Bertin Technologies only in Europe
Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
Product Overview:
HDACs catalyze the hydrolytic removal of acetyl groups from histone lysine residues resulting in chromatin condensation and transcriptional repression of chromosomal DNA. Thus, HDAC inhibition allows the conformation of DNA to be relaxed and transcriptional activation to ensue. Transcriptional repression of key genes has linked HDAC activity to cell growth and tumor development. Cayman’s HDAC Activity Assay provides a fast, fluorescence-based method for measuring Class I and II HDAC activity that eliminates radioactivity, extraction, or chromatography. The procedure requires only two easy steps, both performed in the same microplate. The fluorescent reaction product is analyzed using a plate reader with excitation wavelengths between 340-360 nm and emission wavelengths between 440-465 nm.
Histone deacetylases (HDACs) catalyze the hydrolytic removal of acetyl groups from histone lysine residues resulting in chromatin condensation and transcriptional repression of chromosomal DNA. Thus, HDAC inhibition allows the conformation of DNA to be relaxed and transcriptional activation to ensue. Transcriptional repression of key genes has linked HDAC activity to cell growth and tumor development. Cayman’s HDAC Activity Assay Kit provides a fast, fluorescence-based method for measuring Class I and II HDAC activity that eliminates radioactivity, extraction, or chromatography. The procedure requires only two easy steps, both performed in the same microplate. The fluorescent reaction product is analyzed using a plate reader with excitation wavelengths of 340-360 nm and emission wavelengths of 440-465 nm.
Size: 96 wells
Shipping: dry ice
Stability: Store at -80 degrees; shelf life 365 days maximum after production
Custom Code: 3822000002
UNSPSC code: 41116104

Kwon, H.J., Kim, M.S., Kim, M.J., et al. Histone deacetylase inhibitor FK228 inhibits tumor angiogenesis. Int J Cancer 97 290-296 (2002 Jan 1).

Suenaga, M., Soda, H., Oka, M., et al. Histone deacetylase inhibitors suppress telomerase reverse transcriptase

Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009

Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.

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