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Nitrate/Nitrite Fluorometric Assay Kit

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    Nitrate/<wbr/>Nitrite Fluorometric Assay Kit
  • Nitrate/<wbr/>Nitrite Fluorometric Assay Kit
Cat No: 780051
Assay Kits - Detection Kits

Nitric Oxide (NO) is produced in trace quantities by neurons, endothelial cells, platelets, and neutrophils in response to homeostatic stimuli. This NO is scavenged rapidly (t½ = 4 s) and acts in a paracrine fashion to transduce cellular signals. NO i...

: 2 x 96 wells

This product can only be bought through Cayman Chemical. Please contact us.

Territorial Availability: Available through Bertin Technologies only in France
Correlated keywords:
  • kitrecommendation:no2no3 kits assays nitric oxide synthases reductases fluorescence DAN diaminonaphtalene NO2/NO3 analysistools:flno2no3 fluorescent fluorometric
Product Overview:
Nitric Oxide (NO) is produced in trace quantities by neurons, endothelial cells, platelets, and neutrophils in response to homeostatic stimuli. This NO is scavenged rapidly (t½ = 4 s) and acts in a paracrine fashion to transduce cellular signals. NO interacts with the HEME prosthetic group of guanylate cyclase, activating the enzyme and leading to increased cGMP levels. Other cells (macrophages, fibroblasts, and hepatocytes) also produce NO in micromolar concentrations in response to inflammatory or mitogenic stimuli. These very high levels of NO lead to the formation of peroxynitrite, destruction of iron-sulphur clusters, thiol nitrosation, and nitration of protein tyrosine residues. Thus, the amount of NO produced in different biological systems can vary over several orders of magnitude and its subsequent chemical reactivity is diverse. The final products of NO in vivo are nitrite (NO2-) and nitrate (NO3-). Relative proportions of NO2- and NO3- within a sample are variable and cannot be predicted with certainty. Therefore, the best index of total NO production is the sum of both of these products. Cayman Chemical’s Nitrate/Nitrite Fluorometric Assay Kit provides a convenient method for the quantitation of low levels of NO2- and NO3- in biological samples (particularly tissue culture medium). Previously undetectable concentrations of nitrate/nitrite in a variety of samples can be accurately quantitated using this kit as it extends the lower limit of detection by 20-fold over our popular colorimetric version. The minimum detectable quantity of NO2/NO3 is ~50 nM.
Size 2 x 96 wells
Shipping dry ice
Custom Code 3822.19
UNSPSC code 41116104

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Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

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