Biotin-HPDP

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Biotin-HPDP

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Cat No: 16459

Biochemicals - Labeling & Detection

Biotin-HPDP is a sulfhydryl-reactive biotinylation reagent that forms a reversible disulfide linkage. It is used to label protein cysteines and other substrates that contain sulfhydryl groups.{26858,26856,26855} Biotin-HPDP is also used in the biotin ...

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Territorial Availability: Available through Bertin Technologies only in France

Synonyms:

(3aS,4S,6aR)-hexahydro-2-oxo-N-[6-[[1-oxo-3-(2-pyridinyldithio)propyl]amino]hexyl]-1H-thieno[3,4-d]imidazole-4-pentanamide

Correlated keywords:

immunofluorescences immunocytochemistry immunohistochemistry proteins bindings biochemicals biotins HPDPs sulfhydryl-reactive sulfhydryl reactive biotinylations reagents reversible disulfides linkages disulfide-linkages labels proteins cysteines substrates sulfhydryl groups switches techniques tags S-nitrosylated S nitrosylated SNOs reductions reduces groups thiols tagged interactions interacts avidly streptavidin-coupled streptavidin coupled beads fluorophores enzymes PBS avidin cleaved cleaves reducing agents reducing-agents dithiothreitol EZ-Link™ 1322623-74-4 915035-50-6

Product Overview:
Biotin-HPDP is a sulfhydryl-reactive biotinylation reagent that forms a reversible disulfide linkage. It is used to label protein cysteines and other substrates that contain sulfhydryl groups.{26858,26856,26855} Biotin-HPDP is also used in the biotin switch technique to tag S-nitrosylated (SNO) proteins, following reduction of SNO groups to thiols.{12802,23915} Compounds that are tagged with biotin interact avidly with streptavidin-coupled beads, fluorophores, enzymes, etc. The interaction of biotin-HPDP with substrates containing sulfhydryl groups is easily performed at pH 6.5 to 7.5 in buffers such as PBS. The disulfide linkage that is formed between avidin and substrate can later by cleaved by a reducing agent, like dithiothreitol.

Size	5 mg
Shipping	dry ice
CAS Number	129179-83-5
Molecular Formula	C₂₄H₃₇N₅O₃S₃
SMILES	O=C1N[C@@]2([H])[C@@](CS[C@H]2CCCCC(NCCCCCCNC(CCSSC3=CC=CC=N3)=O)=O)([H])N1
Molecular Weight	539,8
Formulation	A crystalline solid
Purity	≥95%
Custom Code	2909.49
UNSPSC code	12352100

Technical File

MSDS

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Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009

Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.