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Insulin (human) ELISA kit

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    Insulin (human) ELISA kit
  • Insulin (human) ELISA kit
Cat No: A05322
Assay Kits - Elisa
Bertin Bioreagent
€355.00
Price is excluding VAT and does not include packaging neither shipping

Insulin in a polypeptide composed of 51 amino acids divided into two chains (A and B) linked by disulfide bonds. Both chains are organized as alpha helix.
Insulin is produced by the beta cells of the pancreatic islets in form of a precursor (prepro-ins...

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: 96 wells

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Territorial Availability: Available worldwide directly through Bertin or your local distributor
Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
Synonyms:
  • Insulin (human) EIA kit
Correlated keywords:
  • measures
  • kit
  • EIA
  • enzyme
  • immunoassays
  • ELISA
  • diabetes
  • spi-bio
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Product Overview:
Insulin in a polypeptide composed of 51 amino acids divided into two chains (A and B) linked by disulfide bonds. Both chains are organized as alpha helix.
Insulin is produced by the beta cells of the pancreatic islets in form of a precursor (prepro-insulin) and is modified to obtain the pro-insulin (peptide signal elimination) and finally insulin (peptide C elimination).
The main function of the insulin is the regulation of the glucose concentration in the organism. The dysregulation of the production of insulin induced many disorders including diabetes.
Size 96 wells
Shipping wet ice
Specificity
Application Media Cell culture supernatant, plasma and serum|No extraction required; inhibitor cocktail to prevent hemolysis consequences
Sample volume 5 µL
Tracer AcetylCholinesterase AChE
Detection Limit 0.3 µIU/mL (11.5 pg/mL)
Standard Curve Range 0.4 µIU/mL - 52 µIU/mL (15.4 pg/mL – 2000 pg/mL)
Custom Code 3822000000
UNSPSC code 41116104

PRODUCT EXPLANATION: ACETYLCHOLINESTERASE MARKER IN ENZYME-IMMUNOASSAYS

Grassi J., Pradelles P. Compounds labelled by the acetylcholinesterase of Electrophorus Electricus. Its preparation process and its use as a tracer or marquer in enzymo-immunological determinations. United States patent, N° 1,047,330. September 10, 1991

Grassi J., Pradelles P. The use of Acetylcholinesterase as a Universal marker in Enzyme-Immunoassays. Proceedings of the Third International Meeting on Cholinesterases, American Chemical Society (1991)

Pradelles P., Grassi J., Maclouf J. Enzyme Immunoassays of Eicosanoids Using Acetylcholinesterase. Methods in Enzymology (1990), vol. 187, 24-34

PRODUCT EXPLANATION: INSULIN

Wilcox G. Insulin and Insulin Resistance. Clin Biochem Rev Vol 26 May 2005

PRODUCT EXPLANATION: KIT DEVELOPMENT

Valentin MA, Ma S, Zhao A, Legay F, Avrameas A. Validation of immunoassay for protein biomarkers: Bioanalytical study plan implementation to support pre-clinical and clinical studies. J Pharm Biomed Anal. (2011) 55(5): 869-877

European Medicines Agency. Guideline on bioanalytical method validation, 21 July 2011

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