Insulin in a polypeptide composed of 51 amino acids divided into two chains (A and B) linked by disulfide bonds. Both chains are organized as alpha helix.
Insulin is produced by the beta cells of the pancreatic islets in form of a precursor (prepro-ins...
|Application Media||Cell culture supernatant, plasma and serum|No extraction required; inhibitor cocktail to prevent hemolysis consequences|
|Sample volume||5 µL|
|Detection Limit||0.3 µIU/mL (11.5 pg/mL)|
|Standard Curve Range||0.4 µIU/mL - 52 µIU/mL (15.4 pg/mL – 2000 pg/mL)|
PRODUCT EXPLANATION: ACETYLCHOLINESTERASE MARKER IN ENZYME-IMMUNOASSAYS
Grassi J., Pradelles P. Compounds labelled by the acetylcholinesterase of Electrophorus Electricus. Its preparation process and its use as a tracer or marquer in enzymo-immunological determinations. United States patent, N° 1,047,330. September 10, 1991
Grassi J., Pradelles P. The use of Acetylcholinesterase as a Universal marker in Enzyme-Immunoassays. Proceedings of the Third International Meeting on Cholinesterases, American Chemical Society (1991)
Pradelles P., Grassi J., Maclouf J. Enzyme Immunoassays of Eicosanoids Using Acetylcholinesterase. Methods in Enzymology (1990), vol. 187, 24-34
PRODUCT EXPLANATION: INSULIN
Wilcox G. Insulin and Insulin Resistance. Clin Biochem Rev Vol 26 May 2005
PRODUCT EXPLANATION: KIT DEVELOPMENT
Valentin MA, Ma S, Zhao A, Legay F, Avrameas A. Validation of immunoassay for protein biomarkers: Bioanalytical study plan implementation to support pre-clinical and clinical studies. J Pharm Biomed Anal. (2011) 55(5): 869-877
European Medicines Agency. Guideline on bioanalytical method validation, 21 July 2011