The protein quantification method using Coomassie Brilliant Blue G-250 dye has been described for the first time in 1975. Three charge forms of the Coomassie Brilliant Blue dye are present in equilibrium at the usual acidic pH of the assay. The red, bl...More
|Standard Curve Range||0,25-5,00 µg/µL|
Bradford, M. M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254 (1976)
Chial HJ, Thompson HB, Splittgerber AG. A spectral study of the charge forms of Coomassie blue G. Anal Biochem. 1993 Mar;209(2):258-66.