Citrullinated Histone H3 (human,

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Citrullinated Histone H3 (human, recombinant)

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Cat No: 17926

Proteins - More Proteins

A nucleosome is the basic repeating unit of chromatin in which 146 base pairs of DNA wrap twice around a histone octamer consisting of two copies of each of the core histones, H2A, H2B, H3, and H4.{17798} The combination of two H2A/H2B dimers and one ...

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Territorial Availability: Available through Bertin Technologies only in France

Correlated keywords:

histones DNA nucleosomes chromatin acetylation methylation phosphorylation H2A H2B H4 H3 PAD4 PAD2 NETs

Product Overview:
A nucleosome is the basic repeating unit of chromatin in which 146 base pairs of DNA wrap twice around a histone octamer consisting of two copies of each of the core histones, H2A, H2B, H3, and H4.{17798} The combination of two H2A/H2B dimers and one H3/H4 tetramer create the nucleosome core.{17953} Histone H3 undergoes many modifications which include acetylation, methylation, and phosphorylation that are important for regulation of gene transcription.{17798} Citrullination is another important post-translational modification that is catalyzed by peptidylarginine deiminase 4 (PAD4) and PAD2. Hypercitrullination of histones leads to the development of neutrophil extracellular traps (NETs), which are a part of the inflammatory response.{28015,28019} Neutrophils release these NETs to trap and eradicate bacteria and fungi. Failure to clear citrullinated proteins and NET components following inflammation can result in the production of autoantibodies and anti-citrullinated protein antibodies.{28017} The persistence of these antibodies and citrullinated proteins is associated with a number of human diseases including rheumatoid arthritis, systemic lupus erythematosus, Alzheimer’s disease, and multiple sclerosis.{28018} This product contains purified Histone H3 (human, recombinant) (Item No. 10263) that has been modified with PAD4 enzyme, which is removed by affinity chromatography after the reaction.

Size	100 µg
Shipping	dry ice
Application(s)	Immunoreactive with anti-citrulline antibodies by Western blot and ELISA
Formulation	A solution in PBS, pH 7.4
Purity	≥95% estimated by SDS-PAGE
Custom Code	3504.00
UNSPSC code	12352204

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Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009

Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.