QRFP-26RFa (human) ELISA kit

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Cat No: A05037

Assay Kits - Elisa

Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...

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                        Territorial Availability: Available worldwide directly through Bertin or your local distributor
                    

                                            Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
                    

Synonyms:

QRFP-26

RP11-618A20.5

MGC119794

P518

precursor protein

RF(Arg-Phe)amide family

26 amino acid peptide

RF(Arg-Phe)amide family 26 amino acid peptide

Correlated keywords:

ELISA

Obestatin

preproghrelin peptide

Acyl ghrelin

desacyl ghrelin

Insulin

CGRP

Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
The QRFP-26RFa is a neuropeptide of 26 amino acids from the RFamides family (neuropeptide with an arginine and an amidated phenylalanine-motif at its C-end).
The QRFP-26RFa has been discovered in 2003 in European green frog brain. 26RFa has, since then, been characterised in many species such as human, rat and chicken. The structural analysis of the peptide exhibits an amphipathic α-helix in its central domain that plays a role in the interaction with its receptor called GPR103. The QRFP-26RFa is mainly produced in the hypothalamus and in some peripheral tissues like brainstem or the lateral horns of the spinal cord. In vivo studies in mice demonstrated that the QRFP-26RFa is an orexigenic peptide by action on the NPY/proopiomelanocortin system and by inhibition of the glucose-induced insulin secretion. QRFP-26RFa has an action on the regulation of the high fat diet and on the lipolysis in adipocyte of obese individual. In addition, some researchers study the roles of the 26Rfa in sleep and blood pressure regulation.

Size	96 wells
Shipping	dry ice
Application Media	Plasma with extraction on C18 SEP Pack 3cc cartridge
Sample volume	50 µL
Tracer	AcetylCholinesterase AChE
Detection Limit	0,07 ng/mL
Standard Curve Range	0,10-12,50 ng/mL
Custom Code	3822000000
UNSPSC code	41116104

PRODUCT EXPLANATION: ACETYLCHOLINESTERASE MARKER IN ENZYME-IMMUNOASSAYS

Grassi J., Pradelles P. Compounds labelled by the acetylcholinesterase of Electrophorus Electricus. Its preparation process and its use as a tracer or marquer in enzymo-immunological determinations. United States patent, N° 1,047,330. September 10, 1991

Grassi J., Pradelles P. The use of Acetylcholinesterase as a Universal marker in Enzyme-Immunoassays. Proceedings of the Third International Meeting on Cholinesterases, American Chemical Society (1991)

Pradelles P., Grassi J., Maclouf J. Enzyme Immunoassays of Eicosanoids Using Acetylcholinesterase. Methods in Enzymology (1990), vol. 187, 24-34

PRODUCT EXPLANATION : QRFP26-RFa

Chartrel N., Alonzeau J. Alexandre D. et al. The RFamide neuropeptide QRFP-26RFa and its role in the control of neuroendocrine functions. Frontiers in Neuroendocrinology 32 (2011) 387–397

Thuau R., Guilhaudis L., Ségalas-Milazzo I. et al. Structural studies on QRFP-26RFa, a novel human RFamiderelated peptide with orexigenic activity. Peptides 26 (2005) 779–789

Jiang FY., Luo L., Gustafson EL. et al. Identifi cation and characterization of a novel RF-amide peptide ligand for orphan G-protein-coupled receptor SP9155. J. Biol. Chem.278 (2003) 27652–27657

Bruzzone F., Lectez B., Tollemer H. et al. Anatomical distribution and biochemical characterization of the novel RFamide peptide QRFP-26RFa in the human hypothalamus and spinal cord. J. Neurochem. 99 (2006) 616–627

Takayasu S., Sakurai T., Iwasaki S. et al. Neuropeptide ligand of the G protein-coupled receptor GPR103 regulates feeding, behavioral arousal, and blood pressure in mice. Proc. Natl. Acad. Sci.USA 103 (2006) 7438–7443

Egido E.M., Hernández R., Leprince J. et al. QRFP-26RFa, a novel orexigenic neuropeptide, inhibits insulin secretion in the rat pancreas. Peptides 28 (2007) 725–730

Prévost G., Jeandel L., Arabo A. et al. Hypothalamic Neuropeptide 26RFa Acts as an Incretin to Regulate Glucose Homeostasis. Diabetes 2015;64:2805–2816

DOI: 10.2337/db14-1864

Mulumba M., Jossart C., Granata R. et al. GPR103b functions in the peripheral regulation of adipogenesis. Mol. Endocrinol. 24 (2010) 1615–1625

Chen A., Chiu C N., Mosser E A. et al. QRFP and Its Receptors Regulate Locomotor Activity and Sleep in Zebrafish. The Journal of Neuroscience. 2016 February 10, 36(6):1823–1840

PRODUCT EXPLANATION: KIT DEVELOPMENT

Valentin MA, Ma S, Zhao A, Legay F, Avrameas A. Validation of immunoassay for protein biomarkers: Bioanalytical study plan implementation to support pre-clinical and clinical studies. J Pharm Biomed Anal. (2011) 55(5): 869-877

European Medicines Agency. Guideline on bioanalytical method validation, 21 July 2011

Technical File

MSDS