Histamine ELISA kit

Assay Kits - ELISA

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Cat No: A05890

Assay Kits - Elisa

€498.00

Price is excluding VAT and does not include packaging neither shipping

Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...

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                        Territorial Availability: Available worldwide directly through Bertin or your local distributor
                    

                                            Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
                    

Correlated keywords:

measures

allergy

asthma

enzyme

immunoassays

ELISA

fish

mast

cells

allergic

respiratory

diseases

reactions

spi-bio

spibio

A05890-

Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Histamine is a natural amine present in many animal and human tissues. It was considered to be the mediator of immediate allergy. Histamine plays a role in various physiological processes, such as control of gastric acid secretion, neurotransmission and modulation of inflammatory and immunological reactions. This competitive enzyme-immunoassay uses a prederivation (derivate reagent with a structure close to the spacer arm used for the immunogen preparation) of histamine that allows to enhance the sensitivity of the assay.

Size	96 wells
Shipping	Dry ice
Stability	Store at -20degrees; shelf life 1 year maximum after production
Specificity	Histamine: 100% 3 methyl histamine: 0.04% 1 methyl histamine: 0.01% Histidine: <0.01% Serotonin: <0.01%
Application Media	Plasma, urine, culture supernatant as well as liquid (e.g. broncho-alveolar lavage fluids) or solid (brain, nervous tissus) biological samples\|Plasma, urine and culture supernatant: no extraction required\|liquid or solid biological samples: extraction HCI
Sample volume	100 µL
Tracer	AcetylCholinesterase AChE
Detection Limit	0.5 nM
Standard Curve Range	0.4-50 nM
Custom Code	3822000000
UNSPSC code	41116104

PRODUCT EXPLANATION: ACETYLCHOLINESTERASE MARKER IN ENZYME-IMMUNOASSAYS

Grassi J., Pradelles P. Compounds labelled by the acetylcholinesterase of Electrophorus Electricus. Its preparation process and its use as a tracer or marquer in enzymo-immunological

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