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Prostaglandin E Metabolite ELISA Kit

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    Prostaglandin E Metabolite ELISA Kit
  • Prostaglandin E Metabolite ELISA Kit
Cat No: 514531
Assay Kits - Elisa
Price is excluding VAT and does not include packaging neither shipping

Prostaglandin E2 (PGE2) is produced by a variety of cell types which, in general, do not contain the enzymes required for metabolism of PGE2. Thus, cultured endothelial cells or osteoblasts will release PGE2 into the culture medium, where it will accu...


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Territorial Availability: Available through Bertin Technologies only in Europe
Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
Correlated keywords:
  • solid plates kitrecommendation:pge2 bicyclo-PGE2 metabolites 13,14-dihydro-15-keto-PGE2 prostaglandins measures kits eias enzyme immunoassays ELISAs assays
Product Overview:
Prostaglandin E2 (PGE2) is produced by a variety of cell types which, in general, do not contain the enzymes required for metabolism of PGE2. Thus, cultured endothelial cells or osteoblasts will release PGE2 into the culture medium, where it will accumulate without appreciable metabolism. The direct assay of PGE2 from the medium is a good way to measure PGE2 production from these cells. PGE2 is rapidly converted in vivo to its 13,14-dihydro-15-keto metabolite, with more than 90% of circulating PGE2 cleared by a single passage through the lungs. Unfortunately, this metabolite is not chemically stable and undergoes a variable amount of degradation to PGA products. For this reason, blood, urine, or other samples from whole animals or humans often contain very little intact PGE2, and measurement of the metabolites is necessary to provide a reliable estimate of actual PGE2 production. Cayman’s PGE Metabolite assay was developed as a method of converting 13,14-dihydro-15-keto PGA2 and 13,14-dihydro-15-keto PGE2 to a single, stable derivative that could be easily quantified by ELISA. This assay is therefore the method of choice if the samples in question have undergone extensive metabolism prior to collection.
Size 96 strip wells
Shipping dry ice
Stability Store at -20 degrees; shelf life 365 days
Custom Code 3822.00
UNSPSC code 41116104

Hong, J., and Lee, L. Cigarette smoke-induced bronchoconstriction: Causative agents and role of thromboxane receptors. J Appl Physiol 78 2260-2266 (1995).

Muscará, M.N., McKnight, W., Asfaha, S., et al. Wound collagen deposition in rats: Effects of

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