sPLA2 Assay Kit

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sPLA₂ Assay Kit

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Cat No: 765001

Assay Kits - Colorimetric Assays

Phospholipase A2 (PLA2) catalyzes the hydrolysis of phospholipids at the sn-2 position yielding a free fatty acid and a lysophospholipid.{2489} The release of arachidonic acid from membrane phospholipids by PLA2 is believed to be a key step in the con...

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Territorial Availability: Available through Bertin Technologies only in France

Correlated keywords:

phospholipases inflammation eicosanoids colorimetric assays kits sPLA2s enzymes analysistools:spla2

Product Overview:
Phospholipase A2 (PLA2) catalyzes the hydrolysis of phospholipids at the sn-2 position yielding a free fatty acid and a lysophospholipid.{2489} The release of arachidonic acid from membrane phospholipids by PLA2 is believed to be a key step in the control of eicosanoid production within the cell.{1474} The Cayman Chemical secretory PLA2 (sPLA2) Assay kit provides an accurate and convenient method for measurement of sPLA2 activity. This assay uses the 1,2-dithio analog of diheptanoyl phosphatidylcholine which serves as a substrate for most PLA2s (for example, bee and cobra venoms, pancreatic, etc.) with the exception of cytosolic PLA2.{1358,1359} Upon hydrolysis of the thio ester bond at the sn-2 position by PLA2, free thiols are detected using DTNB (5,5-dithio-bis-(2-nitrobenzoic acid)).

Size	96 wells
Shipping	dry ice
Custom Code	3822.19
UNSPSC code	41116104

Hendrickson, H.S., Hendrickson, E.K., and Dybvig, R.H. Chiral synthesis of a dithiolester analog of phosphatidylcholine as a substrate for the assay of phospholipase A₂. J Lipid Res 24 1532-1537 (1983).

Dennis, E.A. Phospholipases. Enzymes XVI

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Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009

Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.