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NETosis Assay Kit

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    NETosis Assay Kit
  • NETosis Assay Kit
Cat No: 601010
Assay Kits - Cell-Based Assays
Cayman
€409.00
Price is excluding VAT and does not include packaging neither shipping

Cayman's NETosis Assay Kit provides a simple and fast method for studying the process of NETosis ex vivo. Notably, Cayman's NETosis Assay Kit does not depend upon the DNA component of neutrophil extracellular traps (NETs), as DNA release can occur ind...

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: 1 ea

This product can only be bought through Cayman Chemical. Please contact us.

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Territorial Availability: Available through Bertin Technologies only in Europe
Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
Correlated keywords:
  • Neutrophil Extracellular Trap kit cell assay measuring measurement measure cell-based CBA neutrophil role engulfment destruction invasive microbe microorganism immune immunity system disease absence dysfunction antimicrobial response trap NET DNA deoxyribonuclease histone macromolecule bacterial plasma DNAse macrophage scavenger receptor-mediated phagocytosis NET/neutrophil complexe component myeloperoxidase MPO elastase NEs lactotransferrin peptide fluorescent fluorescence DNA-intercalating intercalating intercalate dye generation cellular injury injuries necrosis enzyme enzymatic S7 nuclease PMA ionophore A23187 NET-associated associated substrate cleaved cleavages formation NETosis
Product Overview:
Cayman's NETosis Assay Kit provides a simple and fast method for studying the process of NETosis ex vivo. Notably, Cayman's NETosis Assay Kit does not depend upon the DNA component of neutrophil extracellular traps (NETs), as DNA release can occur independently of NETosis. In this kit, primary neutrophils are stimulated to release NETs with either PMA or a calcium ionophore (both included). Unbound neutrophil elastase is washed away following NET generation. Following digest of NET DNA by S7 nuclease, the supernatant containing neutrophil elastase is added to a substrate, which is selectively cleaved by elastase to yield a 4-nitroaniline product that adsorbs light at 405 nm. Enough reagents are provided to test 24 sample conditions for NET production, with analysis in duplicate.
Size 1 ea
Shipping dry ice
Stability Store at -20 degrees; shelf life 180 days
Custom Code 3822.00
UNSPSC code 41116104

Clark, S.R., Ma, A.C., Tavener, S.A., et al. Platelet TLR4 activates neutrophil extracellular traps to ensnare bacteria in septic blood. Nat Med 13(4) 463-469 (2007).

Barrientos, L., Marin-Esteban, V., de Chaisemartin, L., et al. An improved strateg

Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009

Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.

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