Unacylated Ghrelin (dog) Easy Sa

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Unacylated Ghrelin (dog) Easy Sampling ELISA kit

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Cat No: A05320

Assay Kits - Elisa

Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...

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Product Overview
Data
Scientific Literature
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                        Territorial Availability: Available worldwide directly through Bertin or your local distributor
                    

                                            Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
                    

Synonyms:

Unacylated Ghrelin (dog) EIA kit

Des-octanoyl Ghrelin

Correlated keywords:

ELISA

spi-bio

acylated

immunoassays

obesity

growth

hormones

homeostasis

ligands

secretagogue

receptors

stomach

hypothalmic

spi-bio

spibio

adipokines

octanoyl

des-octanoyl

des octanoyl

canine

diabesity

Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Bertin Bioreagent offers a totally new set of assays to measure Acylated & Unacylated Ghrelin, whatever the collection procedure.
Those assays are called Easy Sampling EIA kit because any blood sample collected can be used on any kind of protease inhibitors with or without acidification. From early discovery programs using our 384 wells plate format to select your GOAT inhibitor, to the pre-clinical or clinical stage, Bertin Bioreagent has a solution.

Size	96 wells
Shipping	wet ice
Specificity	refer to technical booklet
Application Media	Plasma, buffer\|Blood collection on inhibitor then dilution prior assay
Tracer	AcetylCholinesterase AChE
Detection Limit	refer to technical booklet
Standard Curve Range	2-250 pg/mL
Custom Code	3822000000
UNSPSC code	41116104

PRODUCT EXPLANATION: ACETYLCHOLINESTERASE MARKER IN ENZYME-IMMUNOASSAYS

Grassi J., Pradelles P. Compounds labelled by the acetylcholinesterase of Electrophorus Electricus. Its preparation process and its use as a tracer or marquer in enzymo-immunological determinations. United States patent, N° 1,047,330. September 10, 1991

Grassi J., Pradelles P. The use of Acetylcholinesterase as a Universal marker in Enzyme-Immunoassays. Proceedings of the Third International Meeting on Cholinesterases, American Chemical Society (1991)

Pradelles P., Grassi J., Maclouf J.Enzyme Immunoassays of Eicosanoids Using Acetylcholinesterase. Methods in Enzymology (1990), vol. 187, 24-34

PRODUCT EXPLANATION: GHRELIN

Kojima M, Kangawa K. Ghrelin: structure and function. Physiol. Rev ( 2005), 85:495-522

Bluet-Pajot MT, Tolle V, Zizzari P, Tomasetto C, Grouselle D, Epelbaum J. Ghrelin: A striking example of neuroendocrine peptide pleiotropy. Med Sci (Paris), August 1, 2005, 21 (8-9): 715-21

Müller TD, Nogueiras R, Andermann ML et al. Ghrelin. Mol Metab. 2015 Mar 21;4(6):437-60

de Faria Barros A et al. Is there association between acyl-ghrelin and inflammation in hemodialysis patients? J Bras Nefrol. (2013) 35(2):120-126.

Grousselle D et al. Variations des peptides dérivés de la préproghréline au cours du repas dans l’anorexie mentale restrictive. Poster GIR-AFDAS-TCA2014

Delhanty P et al. Des-acyl ghrelin analogs prevent high-fat-diet-induced dysregulation of glucose homeostasis. FASEB J. (2013) 27(4):1690-1700

Costantini, V et al. GSK1614343, a Novel Ghrelin Receptor Antagonist, Produces an Unexpected Increase of Food Intake and Body Weight in Rodents and Dogs. Neuroendocrinology (2011);94:158–168

Porporato E, Filigheddu N et al. Acylated and unAcylated Ghrelin impair skeletal muscle atrophy in mice. J. Clinical Invest (2013) 123(2): 611-622

Sentissi O, Epelbaum J, Olié JP, Poirier MF. Leptin and Ghrelin Levels in Patients With Schizophrenia During Different Antipsychotics Treatment: A Review. Schizophrenia Bulletin (2008) 34(6), 1189–1199

PRODUCT EXPLANATION: KIT VALIDATION

Valentin MA, Ma S, Zhao A, Legay F, Avrameas A. Validation of immunoassay for protein biomarkers: Bioanalytical study plan implementation to support pre-clinical and clinical studies. J Pharm Biomed Anal. (2011) 55(5) : 869-877

European Medicines Agency. Guideline on bioanalytical method validation, 21 July 2011.

CITATION OF SPI-BIO DOG GHRELIN KITS

Mitacchione G, Powers JC, Grifoni G et al. The gut hormone ghrelin partially reverses energy substrate metabolic alterations in the failing heart. Circ Heart Fail. 2014 Jul;7(4):643-51. doi: 10.1161/CIRCHEARTFAILURE.114.001167. Epub 2014 May 22.

Technical File

MSDS