TBARS (TCA Method) Assay Kit

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Cat No: 700870

Assay Kits - Colorimetric Assays

Lipid peroxidation is a well-established mechanism of cellular injury in both plants and animals and is used as an indicator of oxidative stress in cells and tissues. Lipid peroxides, derived from PUFAs, are unstable and decompose to form a complex se...

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Territorial Availability: Available through Bertin Technologies only in France

Correlated keywords:

kits assays measures measurements screening screens MDA oxidative stress malondialdehyde lipids peroxidations cells cellular injury injuries plants animals indicates indicators oxidative stress tissues peroxides PUFAs polyunsaturated fatty acids reactive carbonyls compounds human platelets thromboxanes synthases TXS catalyzes prostaglandins PGH2 H2 A2 TXA2 12(S)-HHTrEs Thiobarbituric Reactive Substances fluids drugs foods lipoproteins fractions precipitates precipitated samples saturated unsaturated plasma urine homogenates blood lysates MDA-TBA adducts acidic conditions colorimetrically fluorometrically colorimetric fluorometric NASH non-alcoholic nonalcoholic steatohepatitis NAFLD nonalcoholic fatty liver disease

Product Overview:
Lipid peroxidation is a well-established mechanism of cellular injury in both plants and animals and is used as an indicator of oxidative stress in cells and tissues. Lipid peroxides, derived from PUFAs, are unstable and decompose to form a complex series of compounds, which include reactive carbonyl compounds, such as malondialdehyde (MDA). MDA can be quantified through a controlled reaction with thiobarbituric acid, generating 'Thiobarbituric Acid Reactive Substances' (TBARS). Cayman’s TBARS Assay Kit provides a simple, reproducible, and standardized tool for assaying lipid peroxidation in plasma, serum, urine, tissue homogenates, and cell lysates. The MDA-TBA adduct formed by the reaction of MDA and TBA under high temperature (90-100°C) and acidic conditions can be measured either colorimetrically at 530-540 nm or with much higher sensitivity fluorometrically at an excitation wavelength of 530 nm and an emission wavelength of 550 nm.

Size	96 wells
Shipping	wet ice
Custom Code	3822.19
UNSPSC code	41116104

Jacob, R.A., Aiello, G.M., Stephensen, C.B., et al. Moderate antioxidant supplementation has no effect on biomarkers of oxidant damage in healthy men with low fruit and vegetable intakes. J Nutr 133 740-743 (2003).

Richard, M., Portal, B., Meo, J., et al.

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Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009

Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.