Glutathione S-Transferase Assay

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Glutathione S-Transferase Assay Kit

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Cat No: 703302

Assay Kits - Colorimetric Assays

Glutathione S-transferases (GSTs) are ubiquitous multifunctional enzymes, which play a key role in cellular detoxification. The enzymes protect cells against toxicants by conjugating them to glutathione, thereby neutralizing their electrophilic sites,...

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Territorial Availability: Available through Bertin Technologies only in France

Correlated keywords:

GST assays kits S-transferases GSH activity detoxification antioxidants oxidative injury stress enzymes analysistools:gshases

Product Overview:
Glutathione S-transferases (GSTs) are ubiquitous multifunctional enzymes, which play a key role in cellular detoxification. The enzymes protect cells against toxicants by conjugating them to glutathione, thereby neutralizing their electrophilic sites, and rendering the products more water-soluble.{8335} The glutathione conjugates are metabolized further to mercapturic acid and then excreted.{8335} Based on their sequence homology, substrate specificity, and immunological cross-reactivity, GSTs have been grouped into species-independent classes of isozymes.{8202,7934,8203,8387} These classes are comprised of cytosolic enzymes and the fifth form is microsomal.{8202,7934,8203,8387} The Cayman Chemical Glutathione S-Transferase Assay Kit measures total GST activity (cytosolic and microsomal) by measuring the conjugation of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione.{7935} The conjugation is accompanied by an increase in absorbance at 340 nm. The rate of increase is directly proportional to the GST activity in the sample. The Cayman GST Assay Kit can be used to measure GST activity in plasma, erythrocyte lysates, tissue homogenates, and cell lysates. Cytosolic and microsomal GST activity can also be assayed separately.

Size	96 wells
Shipping	dry ice
Custom Code	3822.19
UNSPSC code	41116104

Mannervik, B., Ålin, P., Guthenberg, C., et al. Identification of three classes of cytosolic glutathione transferase common to several mammalian species: Correlation between structural data and enzymatic properties. Proc Natl Acad Sci USA 82 7202-7206 (19

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Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009

Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.