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HIF-1α Transcription Factor Assay Kit

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    HIF-<wbr/>1α Transcription Factor Assay Kit
  • HIF-<wbr/>1α Transcription Factor Assay Kit
Cat No: 10006910
Assay Kits - Elisa
Cayman
€561.00
Price is excluding VAT and does not include packaging neither shipping

The HIF (hypoxia-inducible factor) transcription factor is a member of the basic-helix-loop-helix (bHLH) family of transcription factors and plays an important role in maintaining cellular oxygen homeostasis.{11044,12428} HIF-1α has emerged as an impo...

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: 96 wells

This product can only be bought through Cayman Chemical. Please contact us.

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Territorial Availability: Available through Bertin Technologies only in Europe
Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
Correlated keywords:
  • tf HIF-1alpha factors HIF1.alpha. cancers basic-helix-loop-helix bHLH cellular oxygen homeostasis cells tissues ischemia infections embryos tumors drugs target breast prostate cardiovascular diseases nuclear extracts whole lysates assays kits ELISAs enzyme-linked immunosorbent double stranded dsDNA HIF1 HIF1alpha HIF1 alpha HIF-1 alpha
Product Overview:
The HIF (hypoxia-inducible factor) transcription factor is a member of the basic-helix-loop-helix (bHLH) family of transcription factors and plays an important role in maintaining cellular oxygen homeostasis.{11044,12428} HIF-1α has emerged as an important drug target in breast and prostate cancer, cardiovascular disease, and ischemia.{10485,10724,11835} Cayman’s HIF-1α Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysate. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) consensus sequence containing the HIF-1α response element is immobilized to the wells of a 96-well plate. HIF-1α contained in a nuclear extract or whole cell lysates, binds specifically to the HIF-1α response element. The HIF transcription factor complex is detected by addition of a specific primary antibody directed against HIF-1α. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm.
Size 96 wells
Shipping dry ice
Stability Store at -80 degrees; shelf life 365 days
Custom Code 3822.00
UNSPSC code 41116104

Turner, K.J., Moore, J.W., Jones, A., et al. Expression of hypoxia-inducible factors in human renal cancer: Relationship to angiogenesis and to the von Hippel-Lindau gene mutation. Cancer Res 62 2957-2961 (2002).

Wang, G.L., Jiang, B., Rue, E.

Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

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ISO/IEC 17025:2005
ISO Guide 34:2009

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