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HIF-1? Transcription Factor Assay Kit

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    HIF-<wbr/>1? Transcription Factor Assay Kit
  • HIF-<wbr/>1? Transcription Factor Assay Kit
Cat No: 10006910
Assay Kits - Elisa

The HIF (hypoxia-inducible factor) transcription factor is a member of the basic-helix-loop-helix (bHLH) family of transcription factors and plays an important role in maintaining cellular oxygen homeostasis.{11044,12428} HIF-1α has emerged as an impo...

: 96 wells

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Territorial Availability: Available through Bertin Technologies only in France
Correlated keywords:
  • tf HIF-1alpha factors HIF1.alpha. cancers basic-helix-loop-helix bHLH cellular oxygen homeostasis cells tissues ischemia infections embryos tumors drugs target breast prostate cardiovascular diseases nuclear extracts whole lysates assays kits ELISAs enzyme-linked immunosorbent double stranded dsDNA HIF1 HIF1alpha HIF1 alpha HIF-1 alpha
Product Overview:
The HIF (hypoxia-inducible factor) transcription factor is a member of the basic-helix-loop-helix (bHLH) family of transcription factors and plays an important role in maintaining cellular oxygen homeostasis.{11044,12428} HIF-1α has emerged as an important drug target in breast and prostate cancer, cardiovascular disease, and ischemia.{10485,10724,11835} Cayman’s HIF-1α Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysate. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) consensus sequence containing the HIF-1α response element is immobilized to the wells of a 96-well plate. HIF-1α contained in a nuclear extract or whole cell lysates, binds specifically to the HIF-1α response element. The HIF transcription factor complex is detected by addition of a specific primary antibody directed against HIF-1α. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm.
Size 96 wells
Shipping dry ice
Custom Code 3822.19
UNSPSC code 41116104

Turner, K.J., Moore, J.W., Jones, A., et al. Expression of hypoxia-inducible factors in human renal cancer: Relationship to angiogenesis and to the von Hippel-Lindau gene mutation. Cancer Res 62 2957-2961 (2002).

Wang, G.L., Jiang, B., Rue, E.

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