The main analytical pitfalls in insulin measurement in serum are related to (a) hemolysis, (b) circulating anti-insulin autoantibodies, and, for the past few years, (c) the reactivity (or lack of reactivity) of rapid- or long-acting pharmacological ins...More
This protocol may be used to measure insulin in plasma or serum sample where hemolysis may occur.
To do so, blood samples are collected in tubes containing EDTA. The samples are centrifuged at 1600 g for 20 minutes. Plasmas are collected and kept at -20°C
Chevenne D., Letailleur A., Trivin F., and Porquet D.,Effect of Hemolysis on the Concentration of Insulin in Serum Determined by RIA and IRMA, Clin. Chem., 44: 354 - 356, Feb 1998
Sapin R., Insulin Immunoassays: Fast Approaching 50 Years of Existence and Still Calling for Standardization, Clinical Chemistry 53: 810-812, 2007