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Insulin (mouse, rat) ELISA kit
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Cat No: A05105
Assay Kits
- Elisa
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...
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Territorial Availability: Available worldwide directly through Bertin or your local distributor
Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
Synonyms:
- Insulin (mouse/rat) EIA kit
Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Insulin is a polypeptide hormone with molecular weight of 6,000 Daltons, composed of two peptides chains, A and B, jointed by two cross-linked disulphide bonds and synthesised by the beta cells of the islets of Langherans of the pancreas. Insulin influences most of the metabolic functions of the body. Its best known action is to lower the blood glucose concentration by increasing the rate at which glucose is converted to glycogen in the liver and muscles and to fat in adipose tissue, by stimulating the rate of glucose metabolism and by depressing gluconeogenesis. Focus on hemolysis: Hemolysis interferes with the assay by degrading insulin. To prevent hemolysis consequences, SPI-BIO has an inhibitor cocktail and a procedure available.
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Insulin is a polypeptide hormone with molecular weight of 6,000 Daltons, composed of two peptides chains, A and B, jointed by two cross-linked disulphide bonds and synthesised by the beta cells of the islets of Langherans of the pancreas. Insulin influences most of the metabolic functions of the body. Its best known action is to lower the blood glucose concentration by increasing the rate at which glucose is converted to glycogen in the liver and muscles and to fat in adipose tissue, by stimulating the rate of glucose metabolism and by depressing gluconeogenesis. Focus on hemolysis: Hemolysis interferes with the assay by degrading insulin. To prevent hemolysis consequences, SPI-BIO has an inhibitor cocktail and a procedure available.
| Size | 96 wells |
| Shipping | Dry ice |
| Specificity | |
| Application Media | Plasma and serum|No extraction required; inhibitor cocktail to prevent hemolysis consequences |
| Sample volume | 50 µL |
| Tracer | AcetylCholinesterase AChE |
| Detection Limit | 0.08 ng/mL |
| Standard Curve Range | 0.08-10 ng/mL |
| Custom Code | 3822000000 |
| UNSPSC code | 41116104 |
PRODUCT EXPLANATION: ACETYLCHOLINESTERASE MARKER IN ENZYME-IMMUNOASSAYS
Grassi J., Pradelles P. Compounds labelled by the acetylcholinesterase of Electrophorus Electricus. Its preparation process and its use as a tracer or marquer in enzymo-immunological determinations. United States patent, N° 1,047,330. September 10, 1991
Grassi J., Pradelles P. The use of Acetylcholinesterase as a Universal marker in Enzyme-Immunoassays. Proceedings of the Third International Meeting on Cholinesterases, American Chemical Society (1991)
Pradelles P., Grassi J., Maclouf J. Enzyme Immunoassays of Eicosanoids Using Acetylcholinesterase. Methods in Enzymology (1990), vol. 187, 24-34
PRODUCT EXPLANATION: INSULIN
Wilcox G. Insulin and Insulin Resistance. Clin Biochem Rev Vol 26 May 2005
PRODUCT EXPLANATION: KIT DEVELOPMENT
Valentin MA, Ma S, Zhao A, Legay F, Avrameas A. Validation of immunoassay for protein biomarkers: Bioanalytical study plan implementation to support pre-clinical and clinical studies. J Pharm Biomed Anal. (2011) 55(5): 869-877
European Medicines Agency. Guideline on bioanalytical method validation, 21 July 2011
CITATIONS OF SPI-BIO INSULIN KIT
Mashmoul M, Azlan A, Mohtarrudin N et al. Saffron Extract and Crocin Reduced Biomarkers Associated with Obesity in Rats Fed a High-Fat Diet. Mal J Nutr 23(1): 117 - 127, 2017
Brajkovic S, Ferdaoussi M, Pawlowski V et al. Islet Brain 1 Protects Insulin Producing Cells against Lipotoxicity. J Diabetes Res. 2016;2016:9158562
El-Seweidy MM, El-Sayed Asker M, Atteia HH, Hegazy SMS. Atorvastatin and Fenofibrate Modulate Certain Steroidal Hormones, Vitamin D and Bile Acids in Diabetic Dyslipidemic Rats. International Journal of Pharmaceutical and Clinical Research 2015; 7(6): 426-434
Tallino S, Duffy M, Ralle M et al. Nutrigenomics analysis reveals that copper deficiency and dietary sucrose up-regulate inflammation, fibrosis and lipogenic pathways in a mature rat model of nonalcoholic fatty liver disease. J Nutr Biochem. 2015 Oct;26(10):996-1006
Ammar HI, Sequiera GL, Nashed MB et al. Comparison of adipose tissue- and bone marrow- derived mesenchymal stem cells for alleviating doxorubicin-induced cardiac dysfunction in diabetic rats. Stem Cell Res Ther. 2015 Aug 22;6:148
Vella RE, Pillon NJ, Zarrouki B et al. Ozone exposure triggers insulin resistance through muscle c-Jun N-terminal kinase activation. Diabetes. 2015 Mar;64(3):1011-24
Hadji L, Berger E, Soula H et al. White adipose tissue resilience to insulin deprivation and replacement. PLoS One. 2014 Aug 29;9(8):e106214
Wong TW, Sumiran N, Mokhtar MT, Kadir A. Blood glucose lowering property of water in oral insulin-fed diabetic rats. Pharm Biol. 2012 Nov;50(11):1463-6
Ibrahim MA, Ibrahim SA, Amin EF et al. Selective versus non selective cyclooxygenase inhibitors in high fructose-induced metabolic Syndrome. Egyptian Journal of Basic and Clinical Pharmacology, December 2012, Vol. 2, No.2: 37: 34
Baquié M, St-Onge L, Kerr-Conte J et al. The liver receptor homolog-1 (LRH-1) is expressed in human islets and protects b-cells against stress-induced apoptosis. Human Molecular Genetics, 2011, Vol. 20, No. 14 : 2823–2833
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