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- Insulin (mouse, rat) ELISA kit
Insulin (mouse, rat) ELISA kit
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Cat No: A05105
Assay Kits
- Elisa
€391.00
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...
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Territorial Availability: Available worldwide directly through Bertin or your local distributor
Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
Synonyms:
- Insulin (mouse/rat) EIA kit
Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Insulin is a polypeptide hormone with molecular weight of 6,000 Daltons, composed of two peptides chains, A and B, jointed by two cross-linked disulphide bonds and synthesised by the beta cells of the islets of Langherans of the pancreas. Insulin influences most of the metabolic functions of the body. Its best known action is to lower the blood glucose concentration by increasing the rate at which glucose is converted to glycogen in the liver and muscles and to fat in adipose tissue, by stimulating the rate of glucose metabolism and by depressing gluconeogenesis. Focus on hemolysis: Hemolysis interferes with the assay by degrading insulin. To prevent hemolysis consequences, SPI-BIO has an inhibitor cocktail and a procedure available.
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Insulin is a polypeptide hormone with molecular weight of 6,000 Daltons, composed of two peptides chains, A and B, jointed by two cross-linked disulphide bonds and synthesised by the beta cells of the islets of Langherans of the pancreas. Insulin influences most of the metabolic functions of the body. Its best known action is to lower the blood glucose concentration by increasing the rate at which glucose is converted to glycogen in the liver and muscles and to fat in adipose tissue, by stimulating the rate of glucose metabolism and by depressing gluconeogenesis. Focus on hemolysis: Hemolysis interferes with the assay by degrading insulin. To prevent hemolysis consequences, SPI-BIO has an inhibitor cocktail and a procedure available.
| Size | 96 wells |
| Shipping | Dry ice |
| Stability | Store at -20degrees; shelf life 3 years maximum after production |
| Specificity | |
| Application Media | Plasma and serum|No extraction required; inhibitor cocktail to prevent hemolysis consequences |
| Sample volume | 50 µL |
| Tracer | AcetylCholinesterase AChE |
| Detection Limit | 0.08 ng/mL |
| Standard Curve Range | 0.08-10 ng/mL |
| Custom Code | 3822000000 |
| UNSPSC code | 41116104 |
Compounds labelled by the acetylcholinesterase of Electrophorus Electricus. Its preparation process and it use as a tracer or marquer in enzymo-immunological determinations. Grassi J. & Pradelles Ph. United States patent, N° 1,047,330. September 1
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