Soluble TNF-? (human, recombinan

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Soluble TNF-? (human, recombinant)

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Cat No: 32020

Proteins - More Proteins

TNF-α is a cytokine and a member of the TNF/TNF receptor (TNFR) cytokine superfamily.{54137} TNF-α is produced as a 233-amino acid transmembrane precursor protein from which mature, soluble TNF-α is formed by proteolysis.{54138} Soluble TNF-α is a 157...

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Territorial Availability: Available through Bertin Technologies only in France

Correlated keywords:

Escherichia trans membrane TNF? TNF alpha TNFR TNFSF AP 1 2 p 38 NF?B AP micro environment fibro sarcoma

Product Overview:
TNF-α is a cytokine and a member of the TNF/TNF receptor (TNFR) cytokine superfamily.{54137} TNF-α is produced as a 233-amino acid transmembrane precursor protein from which mature, soluble TNF-α is formed by proteolysis.{54138} Soluble TNF-α is a 157-amino acid polypeptide, cleaved from the precursor protein on the extracellular side of the membrane, that forms bell-shaped homotrimers with the C-termini at the base, each containing three receptor interaction sites.{54139} It is primarily produced by activated macrophages but can also be produced by a variety of other cells, such as T cells, natural killer cells, and osteoblasts.{54139,54140} TNF-α binds to and activates its receptors, TNFR1 and TNFR2, which are associated with intracellular protein complexes that activate caspases to induce cell death, induce p38 MAPK signaling, and initiate NF-κB or AP-1-mediated transcription of immune and inflammatory mediators.{54141} TNF-α promotes inflammation partly by inducing endothelial cells to express adhesion molecules, COX enzymes, and pro-coagulant factors.{54140} Exogenous TNF-α induces death of cancer cells in vitro, as well as disrupts tumor vascularization and induces tumor necrosis in vivo, but it has tumor promoting properties when produced in the cancer microenvironment.{54137,54143} In contrast, it plays a role in resistance to infection, with mice lacking Tnf having an increased susceptibility to certain microbial infections but lacking resistance to leishmania.{54141} Tnf knockout mice are also resistant to certain types of cancer, including chemically induced skin carcinogenesis.{54137} TNF-α increases lung metastases in a mouse model of fibrosarcoma, an effect that can be reduced by an anti-TNF-α antibody. Mice overexpressing Tnf develop an arthritis similar to rheumatoid arthritis in humans.{54142} TNF-α is produced in the inflamed tissues of patients with inflammatory diseases such as rheumatoid arthritis, and neutralizing antibodies to TNF-α reduce the levels of TNF-α in vitro and in mouse models of the disease.{54140} Cayman’s Soluble TNF-α (human, recombinant) protein can be used for cell-based and binding assay applications. This protein consists of 158 amino acids and has a calculated molecular weight of 17.4 kDa.

Size	10 µg
Shipping	dry ice
Formulation	Lyophilized from sterile PBS, pH 7.4
Purity	≥95% estimated by SDS-PAGE
Custom Code	3504.00
UNSPSC code	12352204

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Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009

Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.