DHN-MA Lipid Peroxidation ELISA

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DHN-MA Lipid Peroxidation ELISA kit

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Cat No: A05033

Assay Kits - Elisa

Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...

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                        Territorial Availability: Available worldwide directly through Bertin or your local distributor
                    

                                            Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
                    

Synonyms:

DHNMA

1,4-dihydroxynonane mercapturic acid

Correlated keywords:

measures

enzyme

immunoassays

ELISA

spi-bio

spibio

A05033

1,4-dihydroxynonane

mercapturic

acid

ELISA

DHNMA

DHN-MA

DH-NMA

Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Lipid peroxidation is a well-established mechanism of cellular injury in both plants and animals and is used as an indicator of oxidative stress in cells and tissues.
Measurement of 4-hydroxy-2-nonenal (HNE), an aldehyde formed during the lipid peroxidation process, is one of several accepted biomarkers for lipid peroxidation. 1,4-Dihydroxynonane mercapturic acid (DHN-MA) is the major urinary metabolite of HNE present at physiological levels in rat and human urine. It can be measured without extraction and offers an alternative lipid peroxidation biomarker aside from 8-isoprostane or TBARS. This DHN-MA EIA Kit is a competitive assay that can be used for quantification of DHN-MA in urine samples. The EIA typically displays an IC50 (50% B/B0) of approximately 75 pg/ml and a detection limit (80% B/B0) of approximately 10 pg/ml.

Size	96 wells
Shipping	dry ice
Specificity	DHN-MA (racemic) 100% HNA-MA 30% HNA 7% 1,4-dihydroxyhexane MA 0.6% butan-1-ol-MA <0.1% DHN <0.1% 1,4-dihydroxyoctane MA <0.1% 1-hexanol-MA <0.1% Show more... Show less... HNA-lactone-MA <0.1% 4-HNE hemiacetal-MA <0.1% 1,4-hydroxyundecane MA <0.1% MA (N-acetyl-cysteine)
Application Media	urine (other upon request)\|No extraction required
Tracer	AcetylCholinesterase AChE
Detection Limit	10 pg/mL
Standard Curve Range	7,8-1000 pg/mL
Custom Code	3822000000
UNSPSC code	41116104

PRODUCT EXPLANATION: ACETYLCHOLINESTERASE MARKER IN ENZYME-IMMUNOASSAYS

Grassi J., Pradelles P. Compounds labelled by the acetylcholinesterase of Electrophorus Electricus. Its preparation process and its use as a tracer or marquer in enzymo-immunological determinations. United States patent, N° 1,047,330. September 10, 1991

Grassi J., Pradelles P. The use of Acetylcholinesterase as a Universal marker in Enzyme-Immunoassays. Proceedings of the Third International Meeting on Cholinesterases, American Chemical Society (1991)

Pradelles P., Grassi J., Maclouf J. Enzyme Immunoassays of Eicosanoids Using Acetylcholinesterase. Methods in Enzymology (1990), vol. 187, 24-34

PRODUCT EXPLANATION: DHN-MA

Spickett CM. The lipid peroxidation product 4-hydroxy-2-nonenal: Advances in chemistry and analysis. Redox Biol. 2013 Jan 21;1(1):145-152. eCollection 2013

Pierre F, Peiro G, Taché S, Cross AJ, Bingham SA, Gasc N, Gottardi G, Corpet DE, Guéraud F. New marker of colon cancer risk associated with heme intake: 1,4-dihydroxynonane mercapturic acid. Cancer Epidemiol Biomarkers Prev. 2006 Nov;15(11):2274-9

Peiro G, Alary J, Cravedi JP, Rathahao E, Steghens JP, Guéraud F. Dihydroxynonane mercapturic acid, a urinary metabolite of 4-hydroxynonenal, as a biomarker of lipid peroxidation. Biofactors. 2005;24(1-4):89-96

Zarkovic N. 4-hydroxynonenal as a bioactive marker of pathophysiological processes. Mol Aspects Med. 2003 Aug-Oct;24(4-5):281-91. Review

Uchida K, Szweda LI, Chae HZ, Stadtman ER. Immunochemical detection of 4-hydroxynonenal protein adducts in oxidized hepatocytes. Proc Natl Acad Sci U S A. 1993 Sep 15;90(18):8742-6

Esterbauer H, Schaur RJ, Zollner H. Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes. Free Radic Biol Med. 1991;11(1):81-128. Review

PRODUCT EXPLANATION: DHN-MA KIT DEVELOPMENT

Guéraud F, Peiro G, Bernard H, Alary J, Créminon C, Debrauwer L, Rathahao E, Drumare MF, Canlet C, Wal JM, Bories G. Enzyme immunoassay for a urinary metabolite of 4-hydroxynonenal as a marker of lipid peroxidation. Free Radic Biol Med. 2006 Jan 1;40(1):54-62. Epub 2005 Sep

Valentin MA, Ma S, Zhao A, Legay F, Avrameas A. Validation of immunoassay for protein biomarkers: Bioanalytical study plan implementation to support pre-clinical and clinical studies. J Pharm Biomed Anal. (2011) 55(5) : 869-877

European Medicines Agency. Guideline on bioanalytical method validation, 21 July 2011

Technical File

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