Vitamin D ELISA Kit

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Vitamin D ELISA Kit

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Cat No: 501050

Assay Kits - Elisa

The primary synthesis of functional vitamin D begins in the skin, where a cholesterol by-product acted upon by UV light forms vitamin D3. Vitamin D3 is carried through the bloodstream, attached to the vitamin D binding protein, to the liver where it i...

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Territorial Availability: Available through Bertin Technologies only in France

Correlated keywords:

kits assays measures measurements EIAs ELISAs enzymes immunoassays immunosorbents vitamins functions regulates intestines intestinal absorptions calcium Ca2+ phosphorus P bones growths immune immunity cells communications extra-skeletons extra skeletal skeletons effects cardiovascular endocrines systems central nervous CNS functional synthesis syntheses skins cholesterols by-products by products 7-dehydrocholesterol 7 dehydro UV ultaviolet lights D3 3 cholecalciferol bloodstreams binding proteins livers 25-hydroxy 25 hydroxy 25-OH OH 25(OH)D3 calcidiol converts conversions converted kidneys 1,25-dihydroxycholecalciferol 1,25 dihydroxy 1,25-(OH)2 (OH)2 1,25(OH)2D3 calcitriol active forms short-lived short lived metabolized metabolizes metabolizing deactivated forms 24,25 dihydroxy 24,25-(OH)2 24,25(OH)2D3 1,24,25 trihydroxy 1,24,25-(OH)3 (OH)3 1,24,25(OH)3D3 diets nutritions dietary supplements activations yeasts fungus fungi ergocalciferol produces biosynthetic routes biologically active plasmas serums detects detections metabolisms metabolically stable displaced displacements humans competitives quantifications quantifying quantifies determination measurements

Product Overview:
The primary synthesis of functional vitamin D begins in the skin, where a cholesterol by-product acted upon by UV light forms vitamin D3. Vitamin D3 is carried through the bloodstream, attached to the vitamin D binding protein, to the liver where it is converted to 25-hydroxy vitamin D3 (25(OH)D3). This product is then transported to the kidney where it is further converted to 1,25-dihydroxycholecalciferol (1,25(OH)2D3), the biologically active form. Active 1,25(OH)2D3 is very short-lived and is rapidly metabolized to its deactivated forms. Vitamin D Immunoassay: Accurate Vitamin D measurement in plasma or serum Cayman’s Vitamin D EIA Kit is a competitive assay that can be used for Vitamin D quantification in plasma or serum. Because of the short half-life of the biologically active 1,25(OH)D form, this Vitamin D assay primarily detects the more metabolically stable forms, 25(OH)D3 and 25(OH)D2. Accurate detection of these forms requires that they be displaced from the vitamin D binding protein prior to measurement. A simple, acetone-based purification protocol is included as the initial procedure of the Vitamin D assay kit for this purpose.

Size	96 solid wells
Shipping	dry ice
Custom Code	3822.19
UNSPSC code	41116104

Gröber, U., Spitz, J., Reichrath, J., et al. Vitamin D: Update 2013: From rickets prophylaxis to general preventive healthcare. Dermatoendocrinol 5(3) 331-347 (2013).

Romagnoli, E., Pepe, J., Piemonte, S., et al. Management of endocrine disease: Value and

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Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009

Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.