CFSE Cell Division Assay Kit

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Cat No: 10009853

Assay Kits - Cell-Based Assays

Cayman’s CFSE Cell Division Assay Kit provides an easy-to-use format for labeling and tracing cells through successive cell divisions. Carboxyfluorescein diacetate, succinimidyl ester (CFDA-SE) diffuses into cells, where the acetate groups are cleaved...

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Territorial Availability: Available through Bertin Technologies only in France

Correlated keywords:

derivative carboxyfluorescein diacetate, succinimidyl ester CFDA-SE cells proliferation growth factors receptors surfaces signaling molecules transcription factors DNA deoxyribonucleases proteins divisions cascades human pathological diseases cancers cell-tracing fluorescent fluorescence dyes proliferative activity parent generations daughter acetate groups molecules cleaved background unstained staining CFSE-labeled radioactivity radioactive labeling divisions inductions inhibitors inhibits inhibitions in vitro models flow cytometry fluorochromes fluorescein

Product Overview:
Cayman’s CFSE Cell Division Assay Kit provides an easy-to-use format for labeling and tracing cells through successive cell divisions. Carboxyfluorescein diacetate, succinimidyl ester (CFDA-SE) diffuses into cells, where the acetate groups are cleaved to yield a highly fluorescent derivative (CFSE) that is retained in the cell and can be detected by flow cytometry. Cell division results in sequential halving of fluorescence, and up to eight divisions can be monitored before the fluorescence is decreased to the background fluorescence of unstained cells.{17802,17820} The ease of use and lack of cytotoxicity allows monitoring of cellular division over weeks either in vitro or in vivo. The kit contains sufficient reagents for labeling and analyzing up to 7.5x108 cells by flow cytometry. CFSE-labeled cells can be further stained with any fluorochrome compatible with fluorescein for use in flow cytometry.

Size	100 tests
Shipping	dry ice
Custom Code	3822.19
UNSPSC code	41116104

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Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009

Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.