Adipolysis Assay Kit

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Cat No: 10009381

Assay Kits - Cell-Based Assays

Cayman's Adipolysis Assay Kit provides a complete assay for studying the lipolysis of triglycerides, from the differentiation of adipocyte-like cells to the quantitation of released glycerol. 3T3-L1 cells are differentiated using a common adipogenesi...

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Territorial Availability: Available through Bertin Technologies only in France

Correlated keywords:

obese obesity cells cycle differentiation inhibits inhibitors inhibition adipocytes cancers origin 3T3-L1 model drug screening adipokines lipolysis 3T3L1 kits assays FFA adipocytes endocrinology cell-based assay cell based assay cba

Product Overview:
Cayman's Adipolysis Assay Kit provides a complete assay for studying the lipolysis of triglycerides, from the differentiation of adipocyte-like cells to the quantitation of released glycerol. 3T3-L1 cells are differentiated using a common adipogenesis induction procedure to accumulate lipid droplets, and isoproterenol is included as a positive control for release of glycerol from triglycerides. Free glycerol in the cell culture medium is measured by incubation with glycerol kinase, glycerol phosphate oxidase, and horseradish peroxidase in the presence of a colorimetric substrate to generate a chromophore detectable at 540 nm. The amount of glycerol release is correlated with both the amount of stored triglyceride and the degree of lipolysis, making this kit useful for investigators screening compounds affecting either lipid storage or metabolism. The Adipolysis Assay Kit provides enough reagents to assay one 96-well plate of supernatants for glycerol release. The accumulation of lipid droplets can be alternatively assayed with either the Lipid Droplets Fluorescence Assay Kit (Item No. 500001) or the Adipogenesis Assay Kit (Item No. 10006908), and glycerol release alone can be assayed using the Glycerol Cell-Based Assay Kit (Item No. 10011725).

Size	1 ea
Shipping	dry ice
Custom Code	3822.19
UNSPSC code	41116104

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Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009

Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.