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Neutrophil Elastase Activity Assay Kit

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    Neutrophil Elastase Activity Assay Kit
  • Neutrophil Elastase Activity Assay Kit
Cat No: 600610
Assay Kits - Cell-Based Assays

Neutrophil elastase is stored within cytoplasmic azurophilic granules in the neutrophil and released upon stimulation by pathogens where it acts either as free protein or is associated with networks of extracellular traps (NET). Together with other pr...

: 2 x 96 wells

This product can only be bought through Cayman Chemical. Please contact us.

Territorial Availability: Available through Bertin Technologies only in France
Correlated keywords:
  • kits assays measures measurement neutrophils elastases enzymes leukocytes humans blood cytoplasmic cytoplasms azurophilic granules cells cellular stimulation pathogens phorbol myristate acetate PMA excreted excretion exocytosed exocytosis proteins networks of extracellular traps NET proteases released degrades degrading invading immune immunity systems responses expression non-small lung cancers tumors lines U937 HL-60 invasion metastasis insoluble elastins matrix mutations ELA2 genes encoding hereditary neutrophenia inflammatory chronic diseases therapeutics non-fluorescent substrates (Z-Ala-Ala-Ala-Ala)2Rh110 R110 whole regulates regulating releases
Product Overview:
Neutrophil elastase is stored within cytoplasmic azurophilic granules in the neutrophil and released upon stimulation by pathogens where it acts either as free protein or is associated with networks of extracellular traps (NET). Together with other proteases released from activated neutrophils, neutrophil elastase plays a critical role in degrading invading pathogens and thus provides the earliest line of defense in the immune system. It is thought to play a critical role in tumor invasion and metastasis as well as other inflammatory conditions. Cayman’s Neutrophil Elastase Activity Assay kit is designed to be used to study compounds regulating elastase release in neutrophils. The kit employs a specific non-fluorescent elastase substrate, (Z-Ala-Ala-Ala-Ala)2Rh110, which is selectively cleaved by elastase to yield the highly fluorescent compound R110, which can be analyzed with an excitation wavelength of 485 nm and emission wavelength of 525 nm. Reagents needed to isolate neutrophils from whole blood are included in the kit, as is PMA, which is known to stimulate elastase release from neutrophils.
Size 2 x 96 wells
Shipping dry ice
Custom Code 3822.19
UNSPSC code 41116104

Brinkmann, V., Reichard, U., Goosmann, C., et al. Neutrophil extracellular traps kill bacteria. Science 303(5663) 1532-1535 (2004).

Boxer, L.A. Severe congenital neutropenia: Genetics and pathogenesis. Trans Am Clin Climatol Assoc 117 13-32 (2006).


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